Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GPR2/CCR10 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: rabbit-anti-goat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Concentration
10 µg/ml

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Submitted Feb 06 2013

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