Flow Cytometry abreview for Anti-GPR2/CCR10 antibody

Good
Abreviews
Application
Flow Cytometry
Sample
Human Cell (keratinocytes)
Specification
keratinocytes
Preparation
Cell harvesting/tissue preparation method: cells were isolated from skin biopsie followed by cell culture
Sample buffer: PBS/10% human IgG
Fixation
Paraformaldehyde
Permeabilization
Yes - 0,1% Tritonx-100/PBS
Gating Strategy
no gate

Other product details

Dilution
1/25
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: PBS/10% human IgG

Secondary antibody

Name
Non-Abcam antibody was used: rabbit-anti-goat PE
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100

Additional data

Additional Notes
Keratinocytes:

the one half of the cells were stained by surface staining and the other half by intracellular staining.

1 = Isotype control
2 = CCR10 – cell surface expression
3 = CCR10 – cytoplasmic expression

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Submitted Feb 08 2013

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