Western blot abreview for Anti-GPR2/CCR10 antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Keratinocytes)
Loading amount
30 µg
Specification
Keratinocytes
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)

Secondary antibody

Name
Non-Abcam antibody was used: rabbit-anti-goat IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500

Detection

Detection method
chemiluminescence
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Positive control
CCR10 recombinant protein

Additional data

Additional Notes
lanes:
1 = NHKs
2 = HaCats
3 = positive control (CCR10 recombinant protein)

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Submitted Feb 08 2013

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