Anti-GPR56 antibody (ab88700)

Submit a review Q&A (1)
Flow Cytometry - Anti-GPR56 antibody (ab88700)
  • Western blot - Anti-GPR56 antibody (ab88700)
  • Western blot - Anti-GPR56 antibody (ab88700)

Key features and details

  • Mouse polyclonal to GPR56
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GPR56 antibody
    See all GPR56 primary antibodies

  • Description

    Mouse polyclonal to GPR56

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length Human protein

  • Positive control

    • Stomach lysate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Could be involved in cell-cell interactions.

  • Tissue specificity

    Widely distributed with highest levels found in thyroid gland, brain and heart. Expressed in a great number of tumor cells.

  • Involvement in disease

    Polymicrogyria, bilateral frontoparietal (BFPP) [MIM:606854]: A malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination, most severe in the frontoparietal regions. BFPP clinical manifestations include developmental and psychomotor delay, cerebellar and pyramidal signs, truncal ataxia, seizures, hyperreflexia. Polymicrogyria is a heterogeneous disorder, considered to be the result of postmigratory abnormal cortical organization. Note=The disease is caused by mutations affecting the gene represented in this entry.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.

  • Post-translational
    modifications

    The endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 7 transmembrane protein with no EGF like N terminal domains 1 antibody
      • BFPP antibody
      • DKFZp781L1398 antibody
      • EGF TM7 like antibody
      • G protein coupled receptor 56 antibody
      • G-protein coupled receptor 56 antibody
      • GPR 56 antibody
      • Gpr56 antibody
      • GPR56_HUMAN antibody
      • Polymicrogyria bilateral frontoparietal antibody
      • Protein TM7XN1 antibody
      • TM7LN4 antibody
      • TM7XN1 antibody
      • TM7XN1 protein antibody
      see all

    Images

    • Flow Cytometry - Anti-GPR56 antibody (ab88700)
      Flow Cytometry - Anti-GPR56 antibody (ab88700)
      FACS analysis of negative control 293 cells (Black) and GPR56-expressing 293 cells (Green) using ab88700 at 1ug/ml.
    • Western blot - Anti-GPR56 antibody (ab88700)
      Western blot - Anti-GPR56 antibody (ab88700)
      Anti-GPR56 antibody (ab88700) at 1/500 dilution + Human stomach lysate at 50 µg

      Predicted band size: 78 kDa

    • Western blot - Anti-GPR56 antibody (ab88700)
      Western blot - Anti-GPR56 antibody (ab88700)
      All lanes : Anti-GPR56 antibody (ab88700) at 1 µg/ml

      Lane 1 : GPR56-transfected 293T cell lysate
      Lane 2 : non transfected lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 78 kDa

    References (0)

    Publishing research using ab88700? Please let us know so that we can cite the reference in this datasheet.

    ab88700 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dear Sir/Madame, For our study we need to perform flow cytometry on murine Gpr56. Since the human and murine GPR56 protein have 87% homology we are interested in the antibody you are offering (ab88700). However, we would like to know the exact protocol that were used for the flow cytometry analysis show in the datasheet figure, specifically the secondary antibody, the control primary antibody, the antibody dilutions, and whether you have any staining results beside whats shown in the datasheet. Since we are using murine cells, we would like to first check the background signal level of the anti-mouse antibody used. We would really appreciate it if you could provide us small amounts of antibodies for a single analysis. Thank you in advance. Kind regards,

    Read More
    Answer

    Thank you for contacting us.

    Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab88700.

    However, if you are interested in testing ab88700 in Flow Cytometry, I would be happy to offer a discount code to test it in this technique and submit your feedback to us.

    Therefore, I can offer a discount off a future purchase if you buy ab88700 now, test it in Flow Cytometry and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    Reply to this email, letting us know you are interested in testing this product.
    Our scientists will email you an inactive personal discount code for the value of the product.
    Purchase and test the product at the regular price.
    Submit your results, noting your AbTrial discount code in your Abreview.
    Once the Abreview is submitted, the discount code will become active.
    Apply your discount code on your next order to receive that value off.



    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com