Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23908-109] to GPR56 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Product nameAnti-GPR56 antibody [EPR23908-109] - BSA and Azide free
See all GPR56 primary antibodies
DescriptionRabbit monoclonal [EPR23908-109] to GPR56 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC,IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment within Human GPR56 aa 1-350. The exact sequence is proprietary.
Database link: Q9Y653
- Flow cyt: Human peripheral blood mononuclear cells.
This antibody ab274428 [EPR23908-109] is a different clone to ab274429 [EPR23908-115].
ab274428 is the carrier-free version of ab259820. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab274428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionCould be involved in cell-cell interactions.
Tissue specificityWidely distributed with highest levels found in thyroid gland, brain and heart. Expressed in a great number of tumor cells.
Involvement in diseasePolymicrogyria, bilateral frontoparietal (BFPP) [MIM:606854]: A malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination, most severe in the frontoparietal regions. BFPP clinical manifestations include developmental and psychomotor delay, cerebellar and pyramidal signs, truncal ataxia, seizures, hyperreflexia. Polymicrogyria is a heterogeneous disorder, considered to be the result of postmigratory abnormal cortical organization. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
modificationsThe endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.
Cellular localizationCell membrane.
- Information by UniProt
- 7 transmembrane protein with no EGF like N terminal domains 1 antibody
- BFPP antibody
- DKFZp781L1398 antibody
This data was developed using ab259820, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling GPR56 with ab259820 at 1/50 dilution (1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259820. Then stained with anti-CD56 conjugated to BV421.
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab274428 has not yet been referenced specifically in any publications.