Overview

  • Product name
  • Description
    Rabbit polyclonal to GPR73B
  • Host species
    Rabbit
  • Specificity
    This antibody reacts specifically with human GPR73B.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from N terminal region of human GPR73B

Properties

Applications

Our Abpromise guarantee covers the use of ab42805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
IHC-Fr 1/50.
Flow Cyt 1/100.
WB 1/200. Predicted molecular weight: 44 kDa.

Target

  • Function
    Receptor for prokineticin 2. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase.
  • Tissue specificity
    Expressed in the ileocecum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary and brain.
  • Involvement in disease
    Hypogonadotropic hypogonadism 3 with or without anosmia
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B830005M06Rik antibody
    • dJ680N4.3 antibody
    • EG-VEGRF2 antibody
    • G protein coupled receptor 73 like 1 antibody
    • G protein coupled receptor I5E antibody
    • G-protein coupled receptor 73-like 1 antibody
    • GPR73b antibody
    • GPR73L1 antibody
    • GPRg2 antibody
    • HH3 antibody
    • KAL3 antibody
    • PK R2 antibody
    • PK-R2 antibody
    • PKR2 antibody
    • PKR2_HUMAN antibody
    • Prokineticin receptor 2 antibody
    • PROKR2 antibody
    see all

Images

  • ab42805 staining GPR73B in umbilical cord (top) and placental villi (bottom) sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). On the right are negative controls

    Ho: Hofbauer cells; Ec: Endothelial cells; Bv: Blood vessels.

References

This product has been referenced in:
  • Urayama K  et al. Transgenic myocardial overexpression of prokineticin receptor-2 (GPR73b) induces hypertrophy and capillary vessel leakage. Cardiovasc Res 81:28-37 (2009). Read more (PubMed: 18806277) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I have just been informed that the information that I gave yesterday in regards to ab90622 was incorrect. The homology of the chick protein is more than 85% homology with the region of the mouse protein from which the antibody was raised. We would therefore expect this antibody to react with your protein of interest. I'm very sorry for this confusion, an error was made in the alignment of the two proteins. If you are still interested in this antibody I would be happy to offer a testing discount. This would involve you buying ab90622, testing it with your chick GPR73B protein, submitting an Abreview (positive or negative) and you would then be eligible for a free primary antibody of your choice. More information on this offer can be found here: www.abcam.com/collaborationdiscount If you would be interested in this promotion please contact me as a discount code needs to be issues prior to purchasing ab90622. Again, I'm very sorry for any confusion caused in this matter, if you have any further questions please do not hesitate to contact us.

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Answer

I have now received confirmation on the homology of the final antibody ab90622 and unfortunately the sequence similarity with the immunogen is again very poor, we would therefore not expect this antibody to recognise the chick protein either. You may have more luck with searching different suppliers using Biocompare: http://www.biocompare.com/ I'm sorry that I have not been able to give you better news. If you have any further questions, please do not hesitate to ask.

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Answer

Thank you for contacting us. I have looked into the 3 antibodies (ab42805, ab90622, ab99453) that we have against GPR73B to see which ones are most likely to also recognise chick GPR73B. ab42805 and ab99453 are both raised against the N-terminal end of human GPR73B (SwissProt Q8NFJ6), which is significantly different from that of chick (SwissProt number B9W049). I would therefore unfortunately not expect either of these antibodies to react with the chick form of the protein. ab90622 is raised against the C-terminal end of mouse GPR73B (SwissProt Q8K458). This region shares much higher homology with that of the chick protein (around 77%) however, in order to know how likely it is going to be that this antibody will react I will check further with the with the lab where the antibody is produced and get back to you as soon as I can.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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