Overview

  • Product name

  • Description

    Rabbit polyclonal to GPRC6A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GPRC6A aa 403-581.
    Database link: Q5T6X5

  • Positive control

    • WB: HepG2 and HeLa whole cell lysate. IHC-P: Human testis and skeletal muscle tissue. ICC/IF: HepG2 cells.
  • General notes

    Previously labelled as GPCR6A. 

Properties

Applications

Our Abpromise guarantee covers the use of ab236964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritionnal and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.
  • Tissue specificity

    Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detected in thymus, prostate, small intestine, tongue and colon. Isoform 1 and isoform 2 are expressed in kidney at the same level. Isoform 2 is expressed at lower level than isoform 1 in the other tissues.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA86F4.3 antibody
    • G protein coupled receptor 33 antibody
    • G protein coupled receptor family C group 6 member A antibody
    • G-protein coupled receptor family C group 6 member A antibody
    • G-protein coupled receptor GPCR33 antibody
    • GPC6A_HUMAN antibody
    • GPCR 33 antibody
    • GPCR 6A antibody
    • GPCR antibody
    • GPCR33 antibody
    • GPCR6A antibody
    • GPR C6A antibody
    • GPRC 6A antibody
    • gprc6a antibody
    • hGPCR 33 antibody
    • hGPCR33 antibody
    • hGPRC6A antibody
    • OTTHUMP00000017075 antibody
    • Predicted with SOSUI analysis antibody
    • seven transmembrane helix receptor antibody
    see all

Images

  • Paraffin-embedded human testis tissue stained for GPRC6A After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.using ab236964 at 1/400 dilution in immunohistochemical analysis.

  • All lanes : Anti-GPRC6A antibody (ab236964) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human skeletal muscle tissue stained for GPRC6A using ab236964 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GPRC6A (green) using ab236964 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

References

ab236964 has not yet been referenced specifically in any publications.

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