• Product name

  • Description

    Rabbit polyclonal to GPT2
  • Host species

  • Specificity

    This antibody may cross-react with GPT1.
  • Tested applications

    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 360-409 (HPEIKGQLVK LLSVRLCPPV SGQAAMDIVV NPPVAGEESF EQFSREKESV) of Human GPT2 (NP_597700).

  • Positive control

    • Fetal brain lysate.



Our Abpromise guarantee covers the use of ab80947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
  • Tissue specificity

    Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
  • Pathway

    Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAT2 antibody
    • Alanine aminotransferase 2 antibody
    • ALAT2_HUMAN antibody
    • ALT2 antibody
    • Glutamate pyruvate transaminase 2 antibody
    • Glutamic alanine transaminase 2 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) 2 antibody
    • Glutamic pyruvic transaminase 2 antibody
    • Glutamic--alanine transaminase 2 antibody
    • Glutamic--pyruvic transaminase 2 antibody
    • GPT 2 antibody
    • gpt2 antibody
    see all


  • Anti-GPT2 antibody (ab80947) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 58 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

    Gel concentration: 12%
  • ICC/IF image of ab80947 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80947, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

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