Key features and details
- Rabbit polyclonal to GRAF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRAF antibody
See all GRAF primary antibodies
DescriptionRabbit polyclonal to GRAF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human GRAF aa 611-661. The exact sequence is proprietary. (NP_055886.1).
Database link: Q9UNA1
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226177 was affinity purified using an epitope specific to GRAF immobilized on solid support.
Our Abpromise guarantee covers the use of ab226177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86,92 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionGTPase-activating protein for RHOA and CDC42.
Involvement in diseaseDefects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
Sequence similaritiesContains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
- Information by UniProt
- arhgap26 antibody
- FLJ42530 antibody
- GRAF antibody
All lanes : Anti-GRAF antibody (ab226177) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 86,92 kDa
Exposure time: 30 seconds
GRAF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226177 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226177 at 0.4 µg/ml.
Lane 1: ab226177 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226177 has not yet been referenced specifically in any publications.