Overview

  • Product name

  • Description

    Rabbit polyclonal to GRAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human GRAF aa 611-661. The exact sequence is proprietary. (NP_055886.1).
    Database link: Q9UNA1

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 86,92 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    GTPase-activating protein for RHOA and CDC42.
  • Involvement in disease

    Defects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • arhgap26 antibody
    • FLJ42530 antibody
    • GRAF antibody
    • GRAF1 antibody
    • GTPase regulator associated with focal adhesion kinase antibody
    • GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody
    • KIAA0621 antibody
    • oligophrenin 1 like protein antibody
    • Oligophrenin-1-like protein antibody
    • oligophrenin1like protein antibody
    • OPHN1L antibody
    • OPHN1L1 antibody
    • RHG26_HUMAN antibody
    • Rho GTPase activating protein 26 antibody
    • Rho GTPase activating protein26 antibody
    • Rho GTPase-activating protein 26 antibody
    • Rho-type GTPase-activating protein 26 antibody
    see all

Images

  • All lanes : Anti-GRAF antibody (ab226177) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 86,92 kDa


    Exposure time: 30 seconds
  • GRAF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226177 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226177 at 0.4 µg/ml.

    Lane 1: ab226177 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab226177 has not yet been referenced specifically in any publications.

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