Anti-GRAF antibody (ab226177)
Key features and details
- Rabbit polyclonal to GRAF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRAF antibody
See all GRAF primary antibodies -
Description
Rabbit polyclonal to GRAF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human GRAF aa 611-661. The exact sequence is proprietary. (NP_055886.1).
Database link: Q9UNA1 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226177 was affinity purified using an epitope specific to GRAF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 86,92 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 86,92 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
GTPase-activating protein for RHOA and CDC42. -
Involvement in disease
Defects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient. -
Sequence similarities
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. -
Cellular localization
Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures. - Information by UniProt
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Database links
- Entrez Gene: 462160 Chimpanzee
- Entrez Gene: 101926312 Cynomolgus monkey
- Entrez Gene: 101154083 Gorilla
- Entrez Gene: 23092 Human
- Entrez Gene: 100436890 Orangutan
- Entrez Gene: 706084 Rhesus monkey
- Omim: 605370 Human
- SwissProt: Q9UNA1 Human
see all -
Alternative names
- arhgap26 antibody
- FLJ42530 antibody
- GRAF antibody
see all
Images
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All lanes : Anti-GRAF antibody (ab226177) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 86,92 kDa
Exposure time: 30 seconds -
GRAF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226177 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226177 at 0.4 µg/ml.
Lane 1: ab226177 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226177 has not yet been referenced specifically in any publications.