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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Anti-GRAF antibody (ab238883)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (ab238883)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (ab238883)

Key features and details

  • Rabbit polyclonal to GRAF
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-GRAF antibody
    See all GRAF primary antibodies
  • Description

    Rabbit polyclonal to GRAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human GRAF aa 625-814.
    Sequence:

    ESVSSNPNSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTSPLS PSWPMFSAPSSPMPTSSTSSDSSPVRSVAGFVWFSVAAVVLSLARSSLHA VFSLLVNFVPCHPNLHLLFDRPEEAVHEDSSTPFRKAKALYACKAEHDSE LSFTAGTVFDNVHPSQEPGWLEGTLNGKTGLIPENYVEFL


    Database link: Q9UNA1-1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human breast cancer and colon cancer tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab238883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.

Target

  • Function

    GTPase-activating protein for RHOA and CDC42.
  • Involvement in disease

    Defects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
  • Target information above from: UniProt accession Q9UNA1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23092 Human
    • Entrez Gene: 71302 Mouse
    • Omim: 605370 Human
    • SwissProt: Q9UNA1 Human
    • SwissProt: Q6ZQ82 Mouse
    • Unigene: 654668 Human
    • Unigene: 329396 Mouse
    • Alternative names

      • arhgap26 antibody
      • FLJ42530 antibody
      • GRAF antibody
      • GRAF1 antibody
      • GTPase regulator associated with focal adhesion kinase antibody
      • GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody
      • KIAA0621 antibody
      • oligophrenin 1 like protein antibody
      • Oligophrenin-1-like protein antibody
      • oligophrenin1like protein antibody
      • OPHN1L antibody
      • OPHN1L1 antibody
      • RHG26_HUMAN antibody
      • Rho GTPase activating protein 26 antibody
      • Rho GTPase activating protein26 antibody
      • Rho GTPase-activating protein 26 antibody
      • Rho-type GTPase-activating protein 26 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (ab238883)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (ab238883)

      Paraffin-embedded human breast cancer tissue stained for GRAF with ab238883 at a 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (ab238883)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (ab238883)

      Paraffin-embedded human colon cancer tissue stained for GRAF with ab238883 at a 1/100 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab238883? Please let us know so that we can cite the reference in this datasheet.

    ab238883 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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