Product nameAnti-GRAF antibody [EPR8252]
See all GRAF primary antibodies
DescriptionRabbit monoclonal [EPR8252] to GRAF
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GRAF aa 750-850. The exact sequence is proprietary.
- Human fetal heart, HeLa, MCF7 and fetal brain lysates; Human brain tissue.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionGTPase-activating protein for RHOA and CDC42.
Involvement in diseaseDefects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
Sequence similaritiesContains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
- Information by UniProt
- arhgap26 antibody
- FLJ42530 antibody
- GRAF antibody
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling GRAF with ab137085 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-GRAF antibody [EPR8252] (ab137085) at 1/1000 dilution
Lane 1 : Human fetal heart lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 92 kDa