Overview

  • Product name

    Anti-GRAF antibody [EPR8252]
    See all GRAF primary antibodies
  • Description

    Rabbit monoclonal [EPR8252] to GRAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GRAF aa 750-850. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, HeLa, MCF7 and fetal brain lysates; Human brain tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      GTPase-activating protein for RHOA and CDC42.
    • Involvement in disease

      Defects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 Rho-GAP domain.
      Contains 1 SH3 domain.
    • Cellular localization

      Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
    • Information by UniProt
    • Database links

    • Alternative names

      • arhgap26 antibody
      • FLJ42530 antibody
      • GRAF antibody
      • GRAF1 antibody
      • GTPase regulator associated with focal adhesion kinase antibody
      • GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody
      • KIAA0621 antibody
      • oligophrenin 1 like protein antibody
      • Oligophrenin-1-like protein antibody
      • oligophrenin1like protein antibody
      • OPHN1L antibody
      • OPHN1L1 antibody
      • RHG26_HUMAN antibody
      • Rho GTPase activating protein 26 antibody
      • Rho GTPase activating protein26 antibody
      • Rho GTPase-activating protein 26 antibody
      • Rho-type GTPase-activating protein 26 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling GRAF with ab137085 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-GRAF antibody [EPR8252] (ab137085) at 1/1000 dilution

      Lane 1 : Human fetal heart lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : MCF7 cell lysates
      Lane 4 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 92 kDa

    References

    This product has been referenced in:

    • Jiang C  et al. RhoA/ROCK/ARHGAP26 signaling in the eutopic and ectopic endometrium is involved in clinical characteristics of adenomyosis. J Int Med Res 46:5019-5029 (2018). Read more (PubMed: 30387365) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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