Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8252] to GRAF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-GRAF antibody [EPR8252]
See all GRAF primary antibodies
DescriptionRabbit monoclonal [EPR8252] to GRAF
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GRAF aa 750-850. The exact sequence is proprietary.
- Human fetal heart, HeLa, MCF7 and fetal brain lysates; Human brain tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionGTPase-activating protein for RHOA and CDC42.
Involvement in diseaseDefects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
Sequence similaritiesContains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
- Information by UniProt
- arhgap26 antibody
- FLJ42530 antibody
- GRAF antibody
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling GRAF with ab137085 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-GRAF antibody [EPR8252] (ab137085) at 1/1000 dilution
Lane 1 : Human fetal heart lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 92 kDa
ab137085 has been referenced in 1 publication.
- Jiang C et al. RhoA/ROCK/ARHGAP26 signaling in the eutopic and ectopic endometrium is involved in clinical characteristics of adenomyosis. J Int Med Res 46:5019-5029 (2018). PubMed: 30387365