Key features and details
- Rabbit polyclonal to Grainyhead-like protein 1 homolog
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Grainyhead-like protein 1 homolog antibody
DescriptionRabbit polyclonal to Grainyhead-like protein 1 homolog
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1-208 of Human Grainyhead-like protein 1 homolog.
- NT2D1, U-87 MG and MCF7 cells and whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111582 is purified by antigen affinity chromatography.
Our Abpromise guarantee covers the use of ab111582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionTranscription factor. Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed. May play a role in development. May be involved in epidermal differentiation (By similarity). Binds and transactivates the EN1 promoter.
Tissue specificityIsoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Highly expressed in placental JEG-3 cells and at very low levels in non-steroidogenic cells. No expression detected in adrenal NCI-H295A cells.
Sequence similaritiesBelongs to the grh/CP2 family. Grainyhead subfamily.
Developmental stageExpressed in fetal liver and brain.
- Information by UniProt
- Grainyhead-like protein 1 homolog antibody
- grhl1 antibody
- GRHL1_HUMAN antibody
Anti-Grainyhead-like protein 1 homolog antibody (ab111582) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 70 kDa
ab111582 at 1/500 dilution staining Grainyhead-like protein 1 homolog in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
ab111582 has not yet been referenced specifically in any publications.