Overview

  • Product name
    Anti-Gram Positive Bacteria antibody [BDI380]
  • Description
    Mouse monoclonal [BDI380] to Gram Positive Bacteria
  • Host species
    Mouse
  • Specificity
    Reactive with lipoteichoic acid (LTA) of many gram positive bacteria. Reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak). Does not react with Clostridium perfringens.
  • Tested applications
    Suitable for: ICC/IF, Conjugation, ELISAmore details
  • Species reactivity
    Reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak). Does not react with Clostridium perfringens. Not yet tested in other species.
  • Immunogen

    Intact Listeria monocytogenes.

  • General notes

     

    Affinity Constant: Not determined.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure; purified from ascites by Protein A chromotography.
  • Primary antibody notes
    Affinity Constant: Not determined.
  • Clonality
    Monoclonal
  • Clone number
    BDI380
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
Conjugation Use at an assay dependent concentration.

Colloidal gold conjugation.

ELISA 1/20 - 1/200.

Target

  • Relevance
    Gram-positive bacteria are characterized by their blue-violet colour reaction in the Gram-staining procedure. The colour reaction is caused by crystal-violet, the primary Gram-stain dye, complexing with the iodine mordant. When the decolorizer is applied, a slow dehydration of the crystal-violet/iodine complex is observed due to the closing of pores running through the cell wall.
  • Cellular localization
    Whole cell
  • Alternative names
    • Gram +ve bacteria antibody

Protocols

References

This product has been referenced in:
  • Béland P  et al. Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor. Biomed Opt Express 6:2908-22 (2015). Read more (PubMed: 26309755) »
See 1 Publication for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for contacting us.


This antibody has not been tested in agglutination applications and we do not suggest using it in such. Per the lab, most antibodies that do function in agglutination are of low affinity. This antibody was developed to produce high affinity.



The lab has suggested the following:

You could try co-agglutination. In this method, the antibody is first bound to killed Staph aureus bacterial suspension by the interaction of protein A that is naturally on the surface of these bacteria. After binding, the bacteria are washed by centrifugation and then resuspended. Now when antigen is added, the Staph aureus suspension agglutinates.
Another alternative to try is to adsorb the antibody to a suspension of latex particles (0.8 micron). After an absorption period, the latex is washed by centrifugation and resuspended. Now when antigen is added, the latex suspension agglutinates.



We cannot guarantee these methods will work, but it may be worth trying them.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for confirming the catalog number.

I am pleased to provide the following information:

1. I am sorry to confirm that ab20344Gram Positive Bacteria antibody [BDI380]has not been tested with P. acnes.

2. This monoclonal antibody reacts with the cell wall of various gram positive bacteria, so therefore it will not stain intracellular. When using this monoclonal in ELISA or colloidal gold conjugated assays, the samples do not need to be fixed and permeabilize with acetone, only when performing Immunofluorescence assays.

I hope this answers your questions and will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

DISCOUNT CODE: x
Expiration date: ddmm2012

I am very pleased to hear you would like to accept our offer and test ab20344inIHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount

Ich wünsche Ihnen viel Erfolg bei Ihrem Projekt!

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Answer

Thank you for your enquriy.

I am happy to let you know that Anti-Gram Positive Bacteria antibody [BDI380] ab20344 was tested against a pool of S. pneumoniae serotypes 6, 7, 9, 14, 18, 19, and 23. Each serotype was not tested separately.

To our knowledge, neither Anti-Streptococcus pneumoniae antibody [B1399M] ab126795 nor Anti-Gram Positive Bacteria antibody [BDI380] ab20344have been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab126795 and/or ab20344 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody each.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab126795and/or ab20344 in IHC-P. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase ab126795 and/or ab20344either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab126795 and/or ab20344turn out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount

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Answer

Thank you very much for your interest in ab20344.

To our knowledge, ab20344 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buyab20344 now, test it inWB and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab20344 in WB. I will then send a discount code. This code must be issued before purchasing ab20344.


2. Purchase ab20344 either by phone, fax, or online (www.abcam.com).

3. Test it in WB.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab20344 turns out to be unsuitable for WB, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. I am sorry for the delay in getting this information to you.

I have contacted the labs regarding your questions and have assemble the following information for you.

AB35126: This antibody has not been tested with bacterial wall teichoic acid or ribitol teichoic acid. We also have not tested this antibody in immunoprecipitation.

AB20344: MAb to Lipoteichoic Acid, will react with bacterial wall teichoic acid. This antibody has not been tested for reactivity to ribitol teichoic acid.We also have not tested this antibody in immunoprecipitation.



AB21100: This antibody has not been tested for reactivity with bacterial wall teichoic acid or ribitol teichoic acid. This antibody has not been tested for use in immunoprecipitation.



If you should with to test any or all of these products in IP, we could offer you our 100% Abreview discount. Basically a buy-one-get-one free program, the Abreview 100% discount gives you a code redeemable for 1 free primary antibody if you buy an untested product, test it in IP and submit and Abreview. If you are interested I can send you further information.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

DISCOUNT CODE:100%ABR-E7TUT Ablaufdatum: 19.03.2012 Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab20344 in der IHC in Maus zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 100%ABR-E7TUT und kann zum Kauf weiterer Produkte eingesetzt werden. Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in der IHC eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer. Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung. Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Answer

Thank you for your enquiry. I have investiaged this and can confirm that ab20344 Gram Positive Bacteria antibody will stain the cell wall. We have not tested this antibodiy for staining of the capsule, so I am sorry we are not able to provide or confirm any details for this. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry. I can confirm that this product is reactive with lipoteichoic acid (LTA) of many gram positive bacteria. It also cross-reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak). However, it does not react with Clostridium perfringens. I hope this information will be useful. Have a nice day.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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