Anti-Gram Positive Bacteria antibody [BDI380] (ab20344)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Gram Positive Bacteria antibody [BDI380]
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DescriptionMouse monoclonal [BDI380] to Gram Positive Bacteria
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Host speciesMouse
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SpecificityReactive with lipoteichoic acid (LTA) of many gram positive bacteria. Reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak). Does not react with Clostridium perfringens.
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Tested applicationsSuitable for: ICC/IF, Conjugation, ELISAmore details
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Species reactivityReacts with: Other species
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Immunogen
Intact Listeria monocytogenes.
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General notes
Affinity Constant: Not determined.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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PurityAscites
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Purification notesPurified from ascites.
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Primary antibody notesAffinity Constant: Not determined.
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ClonalityMonoclonal
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Clone numberBDI380
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab20344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/10 - 1/50. | |
Conjugation | Use at an assay dependent concentration. Colloidal gold conjugation. |
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ELISA | 1/20 - 1/200. |
Target
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RelevanceGram-positive bacteria are characterized by their blue-violet colour reaction in the Gram-staining procedure. The colour reaction is caused by crystal-violet, the primary Gram-stain dye, complexing with the iodine mordant. When the decolorizer is applied, a slow dehydration of the crystal-violet/iodine complex is observed due to the closing of pores running through the cell wall.
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Cellular localizationWhole cell
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Alternative names
- Gram +ve bacteria antibody
Protocols
References
This product has been referenced in:
- Béland P et al. Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor. Biomed Opt Express 6:2908-22 (2015). Read more (PubMed: 26309755) »