Anti-Gram Positive Bacteria antibody [BDI380] (ab20344)

Submit an Abreview Q&A (9)Specific References (1)

Overview

  • Product name
    Anti-Gram Positive Bacteria antibody [BDI380]
  • Description
    Mouse monoclonal [BDI380] to Gram Positive Bacteria
  • Host species
    Mouse
  • Specificity
    Reactive with lipoteichoic acid (LTA) of many gram positive bacteria. Reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak). Does not react with Clostridium perfringens.
  • Tested applications
    Suitable for: ICC/IF, Conjugation, ELISAmore details
  • Species reactivity
    Reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak). Does not react with Clostridium perfringens. Not yet tested in other species.
  • Immunogen

    Intact Listeria monocytogenes.

  • General notes


    Affinity Constant: Not determined.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure; purified from ascites by Protein A chromotography.
  • Primary antibody notes
    Affinity Constant: Not determined.
  • Clonality
    Monoclonal
  • Clone number
    BDI380
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
Conjugation Use at an assay dependent concentration.

Colloidal gold conjugation.
ELISA 1/20 - 1/200.

Target

  • Relevance
    Gram-positive bacteria are characterized by their blue-violet colour reaction in the Gram-staining procedure. The colour reaction is caused by crystal-violet, the primary Gram-stain dye, complexing with the iodine mordant. When the decolorizer is applied, a slow dehydration of the crystal-violet/iodine complex is observed due to the closing of pores running through the cell wall.
  • Cellular localization
    Whole cell
  • Alternative names
    • Gram +ve bacteria antibody

Protocols

References

This product has been referenced in:
  • Béland P  et al. Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor. Biomed Opt Express 6:2908-22 (2015). Read more (PubMed: 26309755) »

See 1 Publication for this product

Publishing research using ab20344? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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9 reviews or Q&A

10
Votes: Highest First


Inquiry: I have tried doing an agglutination assay on both Bacillus subtilis and Staphylococcus epidermidis and neather worked. I used the antibody mixed 1:1, 1:10 1:100 and 1:1000. The bacteria were grown in LB broth. centrifuged twice both times...

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Thank you for contacting us.


This antibody has not been tested in agglutination applications and we do not suggest using it in such. Per the lab, most antibodies that do function in agglutination are of low affinity. This antibody was dev...

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Dear Sir or Madam,

I am working on Propionibacterium acnes, and interested in your product,
Anti-Gram Positive Bacteria antibody [BDI380] to stain P. acnes which is
residing in human epithelial cells.

I am actually worry...

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Thank you for confirming the catalog number.

I am pleased to provide the following information:

1. I am sorry to confirm that ab20344Gram Positive Bacteria antibody [BDI380]has not been tested with P. acnes.

2. This mon...

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Thank you for your offer!
I would like to try out how the Anti-Gram Positive Bacteria antibody
[BDI380] ab20344 works in our IHC-P setting and will gladly post a review.

DISCOUNT CODE: x
Expiration date: ddmm2012

I am very pleased to hear you would like to accept our offer and test ab20344inIHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please subm...

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Thank you for your reply. It would be great if you could answer a few more questions:

Do you have any information whether the antibody (ab126795) might be suitable for immunohistochemistry (IHC-P)?

I also noticed another antibody ...

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Thank you for your enquriy.

I am happy to let you know that Anti-Gram Positive Bacteria antibody [BDI380] ab20344 was tested against a pool of S. pneumoniae serotypes 6, 7, 9, 14, 18, 19, and 23. Each serotype was not tested separately.
...

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Thanks for the information. Please can you tell me whether product ab20344 has be tested in Western Blotting.

Thank you very much for your interest in ab20344.

To our knowledge, ab20344 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buyab20344 now, test it inWB and submit feedback to us in the form of an Ab...

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Hello, I am interested in the following products: ab35126 Anti Lipoteichoic Acid Antibody ab20344 Anti-Gram positive bacteria antibody ab21100 Anti-lipoteichoic acid antibody Please can you tell me whether these antibodies also react with bact...

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Thank you for contacting us. I am sorry for the delay in getting this information to you.

I have contacted the labs regarding your questions and have assemble the following information for you.

AB35126: This antibody has not been ...

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Vielen Dank für das Angebot. Gerne würde ich den Antikörper ab20344 testen. Bitte schicken Sie mir einen Rabattcode zu.

DISCOUNT CODE:100%ABR-E7TUT Ablaufdatum: 19.03.2012 Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab20344 in der IHC in Maus zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antik...

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Does your product described as Gram Positive Bacteria antibody [BDI380] (ab20344) stain both the capsule and cell wall of the Bacillus sp.? Thanks.

Thank you for your enquiry. I have investiaged this and can confirm that ab20344 Gram Positive Bacteria antibody will stain the cell wall. We have not tested this antibodiy for staining of the capsule, so I am sorry we are not able to provide or co...

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Thank you very much for your prompt reply. In looking at the datasheet for ab20344, it says that the ab reacts with LTA of many gram-positive bacteria. Would it be possible to get a list of the bacteria that this ab has actually been demonstrated to ...

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Thank you for your enquiry. I can confirm that this product is reactive with lipoteichoic acid (LTA) of many gram positive bacteria. It also cross-reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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