Key features and details
- Mouse monoclonal [G43J] to Gram Positive Bacteria
- Suitable for: WB
- Reacts with: Bacillus subtilis
- Isotype: IgG1
Product nameAnti-Gram Positive Bacteria antibody [G43J]
DescriptionMouse monoclonal [G43J] to Gram Positive Bacteria
THis antibody reacts with Gram positive bacteria Lipoteichoic acid (LTA) in bacterial and infected tissue samples.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Bacillus subtilis
Other Immunogen Type corresponding to Gram Positive Bacteria. Lipoteichoic acid consistent with that of gram positive bacteria.
- WB: Bacillus subtilis LTA.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/250.
1/10 - 1/250.
RelevanceGram-positive bacteria are characterized by their blue-violet colour reaction in the Gram-staining procedure. The colour reaction is caused by crystal-violet, the primary Gram-stain dye, complexing with the iodine mordant. When the decolorizer is applied, a slow dehydration of the crystal-violet/iodine complex is observed due to the closing of pores running through the cell wall.
Cellular localizationWhole cell
- Gram +ve bacteria antibody
All lanes : Anti-Gram Positive Bacteria antibody [G43J] (ab267414) at 1/250 dilution
Lane 1 : Wild-type B. subtilis LTA
Lane 2 : Mutant form of B. subtilis lacking LTA
Lysates/proteins at 65 µg per lane.
All lanes : Alkaline phosphatase conjugated secondary antibody and NBT/BCIP
Wild-type B. subtilis or a mutant form of B. subtilis lacking LTA were grown to the same optical density and lysed.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab267414 has not yet been referenced specifically in any publications.