Key features and details
- Rabbit polyclonal to GRAM1DA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRAM1DA antibody
See all GRAM1DA primary antibodies
DescriptionRabbit polyclonal to GRAM1DA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GRAM1DA aa 674-724. The exact sequence is proprietary. (NP_065946.2).
Database link: Q96CP6
- WB: HeLa, HEK-293T, MCF7, Jurkat and K562 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab240586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesContains 1 GRAM domain.
- Information by UniProt
- 1300003M23Rik antibody
- AI415002 antibody
- AW547240 antibody
All lanes : Anti-GRAM1DA antibody (ab240586) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 30 seconds
GRAM1DA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240586 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab240586 at 1 µg/ml.
Lane 1: ab240586 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240586 has not yet been referenced specifically in any publications.