Key features and details
- Rabbit polyclonal to GRAMD1B
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRAMD1B antibody
DescriptionRabbit polyclonal to GRAMD1B
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to internal sequence amino acids 518-624 of Human GRAMD1B.
- Human gall bladder tissue, RT-4 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
Sequence similaritiesContains 1 GRAM domain.
- Information by UniProt
- GRAM domain containing 1B antibody
- GRAM domain-containing protein 1B antibody
- Gramd1b antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab121286 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121286, at a 1/100 dilution, staining GRAMD1B in paraffin-embedded Human gall bladder tissue by Immunohistochemistry.
All lanes : Anti-GRAMD1B antibody (ab121286) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121286 has been referenced in 1 publication.
- Khanna P et al. GRAMD1B regulates cell migration in breast cancer cells through JAK/STAT and Akt signaling. Sci Rep 8:9511 (2018). PubMed: 29934528