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Rabbit polyclonal to GRAMD1B
Predicted to work with:
Recombinant fragment, corresponding to a region within amino acids 375-589 of Human GRAMD1B (Uniprot ID: Q3KR37).
NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; MCF7 cells and Human MCF7 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 85 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Contains 1 GRAM domain.
Information by UniProt
GRAM domain containing 1B antibody
GRAM domain-containing protein 1B antibody
Western blot - Anti-GRAMD1B antibody (ab154934)
Anti-GRAMD1B antibody (ab154934) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 85 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1B antibody (ab154934)
Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling GRAMD1B with ab154934 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1B antibody (ab154934)
Immunofluorescence analysis of methanol-fixed MCF7 cells labeling GRAMD1B with ab154934 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"