Overview

  • Product name
  • Description
    Rabbit polyclonal to GRAMD1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 375-589 of Human GRAMD1B (Uniprot ID: Q3KR37).

  • Positive control
    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; MCF7 cells and Human MCF7 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 85 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-GRAMD1B antibody (ab154934) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 85 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling GRAMD1B with ab154934 at 1/100 dilution.
  • Immunofluorescence analysis of methanol-fixed MCF7 cells labeling GRAMD1B with ab154934 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Khanna P  et al. GRAM domain-containing protein 1B (GRAMD1B), a novel component of the JAK/STAT signaling pathway, functions in gastric carcinogenesis. Oncotarget 8:115370-115383 (2017). Read more (PubMed: 29383166) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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