Key features and details
- Rabbit polyclonal to GRAMD1C
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRAMD1C antibody
DescriptionRabbit polyclonal to GRAMD1C
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Recombinant fragment, corresponding to amino acids 452-541 of Human GRAMD1C (Q8IYS0).
- Human smooth muscle tissue; RT4, U251 MG, Human liver and tonsil lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesContains 1 GRAM domain.
- Information by UniProt
- 4921521N14Rik antibody
- GRAM domain-containing protein 1C antibody
- GRAMD1C antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121365 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121365 at 1/500 dilution staining GRAMD1C in Paraffin-emebedded Human Smooth muscle tissue by Immunohistochemistry.
All lanes : Anti-GRAMD1C antibody (ab121365) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121365 has not yet been referenced specifically in any publications.