Product nameAnti-Granulin antibody
See all Granulin primary antibodies
DescriptionMouse polyclonal to Granulin
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- Granulin transfected 293T cell lysate; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Our Abpromise guarantee covers the use of ab169325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 64 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml. Antibody purification with Protein A will be needed prior to use.|
FunctionGranulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
Tissue specificityIn myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
Involvement in diseaseDefects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
Sequence similaritiesBelongs to the granulin family.
modificationsGranulins are disulfide bridged.
- Information by UniProt
- Acrogranin antibody
- CLN11 antibody
- GEP antibody
All lanes : Anti-Granulin antibody (ab169325) at 1/500 dilution
Lane 1 : Granulin transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 64 kDa
Immunofluorescent analysis of permeabilized HeLa cells labeling Granulin with purified ab169325 at 10µg/ml.
Antibody purification with Protein A will be needed prior to use.
ab169325 has not yet been referenced specifically in any publications.