Overview

  • Product name
  • Description
    Mouse polyclonal to Granulin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Full length protein, corresponding to amino acids 1-593 of Human Granulin (NP_002078).

  • Positive control
    • Granulin transfected 293T cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 64 kDa.
ICC/IF Use a concentration of 10 µg/ml. Antibody purification with Protein A will be needed prior to use.

Target

  • Function
    Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
    Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
  • Tissue specificity
    In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
  • Involvement in disease
    Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
  • Sequence similarities
    Belongs to the granulin family.
  • Post-translational
    modifications
    Granulins are disulfide bridged.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrogranin antibody
    • CLN11 antibody
    • GEP antibody
    • GP88 antibody
    • Granulin A antibody
    • Granulin B antibody
    • Granulin C antibody
    • Granulin D antibody
    • Granulin E antibody
    • Granulin epithelin antibody
    • Granulin F antibody
    • Granulin G antibody
    • Granulin-7 antibody
    • Granulins antibody
    • GRN antibody
    • GRN_HUMAN antibody
    • PC cell derived growth factor antibody
    • PCDGF antibody
    • PEPI antibody
    • PGRN antibody
    • Proepithelin antibody
    • Progranulin antibody
    see all

Images

  • All lanes : Anti-Granulin antibody (ab169325) at 1/500 dilution

    Lane 1 : Granulin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa

  • Immunofluorescent analysis of permeabilized HeLa cells labeling Granulin with purified ab169325 at 10µg/ml.
    Antibody purification with Protein A will be needed prior to use.

References

ab169325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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