Anti-Granulin antibody (ab191211)
Key features and details
- Rabbit polyclonal to Granulin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Granulin antibody
See all Granulin primary antibodies -
Description
Rabbit polyclonal to Granulin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Granulin aa 1-350.
Database link: P28799 -
Positive control
- WB: HL60, PC12, MCF7, THP1, HepG2 and 293T cell line lysates; Mouse kidney, liver and spleen tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa. |
Target
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Function
Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. -
Tissue specificity
In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. -
Involvement in disease
Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. -
Sequence similarities
Belongs to the granulin family. -
Post-translational
modificationsGranulins are disulfide bridged. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2896 Human
- Entrez Gene: 14824 Mouse
- Entrez Gene: 29143 Rat
- Omim: 138945 Human
- SwissProt: P28799 Human
- SwissProt: P28798 Mouse
- SwissProt: P23785 Rat
- Unigene: 514220 Human
see all -
Alternative names
- Acrogranin antibody
- CLN11 antibody
- GEP antibody
see all
Images
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All lanes : Anti-Granulin antibody (ab191211) at 1/1000 dilution
Lane 1 : HL60 cell line lysate
Lane 2 : THP1 cell line lysate
Lane 3 : PC12 cell line lysate
Lane 4 : 293T cell line lysate
Lane 5 : HepG2 cell line lysate
Lane 6 : MCF7 cell line lysate
Lane 7 : Mouse liver tissue lysate
Lane 8 : Mouse spleen tissue lysate
Lane 9 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 5 secondsBlocking buffer: 3% nonfat dry milk in TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab191211 has been referenced in 5 publications.
- Wei F et al. Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling. PLoS Pathog 15:e1008062 (2019). PubMed: 31585000
- Voshtani R et al. Progranulin promotes melanoma progression by inhibiting natural killer cell recruitment to the tumor microenvironment. Cancer Lett 465:24-35 (2019). PubMed: 31491449
- Zhao XA et al. Emodin Alleviates Liver Fibrosis of Mice by Reducing Infiltration of Gr1hi Monocytes. Evid Based Complement Alternat Med 2018:5738101 (2018). PubMed: 29743924
- Xie W et al. miR-34b-5p inhibition attenuates lung inflammation and apoptosis in an LPS-induced acute lung injury mouse model by targeting progranulin. J Cell Physiol 233:6615-6631 (2018). PubMed: 29150939
- Chen L et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . PubMed: 28609022