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    granulin-antibody-ab191211.pdf

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Anti-Granulin antibody (ab191211)

  • Datasheet
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Western blot - Anti-Granulin antibody (ab191211)

    Key features and details

    • Rabbit polyclonal to Granulin
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Recombinant human Granulin protein (ab113403)
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    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Granulin antibody
      See all Granulin primary antibodies
    • Description

      Rabbit polyclonal to Granulin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Recombinant fragment corresponding to Human Granulin aa 20-310.
      Sequence:

      CPDGQFCPVAC CLDPGGASYS CCRPLLDKWP TTLSRHLGGP CQVDAHCSAG HSCIFTVSGT SSCCPFPEAV ACGDGHHCCP RGFHCSADGR SCFQRSGNNS VGAIQCPDSQ FECPDFSTCC VMVDGSWGCC PMPQASCCED RVHCCPHGAF CDLVHTRCIT PTGTHPLAKK LPAQRTNRAV ALSSSVMCPD ARSRCPDGST CCELPSGKYG CCPMPNATCC SDHLHCCPQD TVCDLIQSKC LSKENATTDL LTKLPAHTVG DVKCDMEVSC PDGYTCCRLQ SGAWGCCPFT


      Database link: P28799
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: HL60, PC12, MCF7, THP1, HepG2 and 293T cell line lysates; Mouse kidney, liver and spleen tissue lysates.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Immunology
      • Secreted Molecules
      • Other secreted molecules
      • Cancer
      • Signal transduction
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HepG2 whole cell lysate (ab166833)
      • Mouse liver tissue lysate - total protein (ab29301)
      • Mouse kidney normal tissue lysate - total protein (ab29305)
      • MCF7 whole cell lysate (ab29537)
      • THP1 whole cell lysate (ab7913)
      • HL60 whole cell lysate (ab7914)
      • Mouse spleen tissue lysate - total protein (ab7937)
    • Recombinant Protein

      • Recombinant human Granulin protein (ab113403)

    Applications

    Our Abpromise guarantee covers the use of ab191211 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/2000. Predicted molecular weight: 64 kDa.

    Target

    • Function

      Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
      Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
    • Tissue specificity

      In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
    • Involvement in disease

      Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
    • Sequence similarities

      Belongs to the granulin family.
    • Post-translational
      modifications

      Granulins are disulfide bridged.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P28799 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2896 Human
      • Entrez Gene: 14824 Mouse
      • Entrez Gene: 29143 Rat
      • Omim: 138945 Human
      • SwissProt: P28799 Human
      • SwissProt: P28798 Mouse
      • SwissProt: P23785 Rat
      • Unigene: 514220 Human
      • Unigene: 1568 Mouse
      • Unigene: 5820 Rat
      see all
    • Alternative names

      • Acrogranin antibody
      • CLN11 antibody
      • GEP antibody
      • GP88 antibody
      • Granulin A antibody
      • Granulin B antibody
      • Granulin C antibody
      • Granulin D antibody
      • Granulin E antibody
      • Granulin epithelin antibody
      • Granulin F antibody
      • Granulin G antibody
      • Granulin-7 antibody
      • Granulins antibody
      • GRN antibody
      • GRN_HUMAN antibody
      • PC cell derived growth factor antibody
      • PCDGF antibody
      • PEPI antibody
      • PGRN antibody
      • Proepithelin antibody
      • Progranulin antibody
      see all

    Images

    • Western blot - Anti-Granulin antibody (ab191211)
      Western blot - Anti-Granulin antibody (ab191211)
      All lanes : Anti-Granulin antibody (ab191211) at 1/1000 dilution

      Lane 1 : HL60 cell line lysate
      Lane 2 : THP1 cell line lysate
      Lane 3 : PC12 cell line lysate
      Lane 4 : 293T cell line lysate
      Lane 5 : HepG2 cell line lysate
      Lane 6 : MCF7 cell line lysate
      Lane 7 : Mouse liver tissue lysate
      Lane 8 : Mouse spleen tissue lysate
      Lane 9 : Mouse kidney tissue lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : HRP Goat Anti-Rabbit IgG (H+L)

      Developed using the ECL technique.

      Predicted band size: 64 kDa


      Exposure time: 5 seconds


      Blocking buffer: 3% nonfat dry milk in TBST.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab191211? Please let us know so that we can cite the reference in this datasheet.

    ab191211 has been referenced in 4 publications.

    • Wei F  et al. Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling. PLoS Pathog 15:e1008062 (2019). PubMed: 31585000
    • Xie W  et al. miR-34b-5p inhibition attenuates lung inflammation and apoptosis in an LPS-induced acute lung injury mouse model by targeting progranulin. J Cell Physiol 233:6615-6631 (2018). PubMed: 29150939
    • Zhao XA  et al. Emodin Alleviates Liver Fibrosis of Mice by Reducing Infiltration of Gr1hi Monocytes. Evid Based Complement Alternat Med 2018:5738101 (2018). PubMed: 29743924
    • Chen L  et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . PubMed: 28609022

    Customer reviews and Q&As

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