Anti-Granulin antibody (ab191211)

Submit a review Submit a question References (3)

Overview

  • Product name

    Anti-Granulin antibody
    See all Granulin primary antibodies

  • Description

    Rabbit polyclonal to Granulin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human Granulin aa 20-310.
    Sequence:

    CPDGQFCPVAC CLDPGGASYS CCRPLLDKWP TTLSRHLGGP CQVDAHCSAG HSCIFTVSGT SSCCPFPEAV ACGDGHHCCP RGFHCSADGR SCFQRSGNNS VGAIQCPDSQ FECPDFSTCC VMVDGSWGCC PMPQASCCED RVHCCPHGAF CDLVHTRCIT PTGTHPLAKK LPAQRTNRAV ALSSSVMCPD ARSRCPDGST CCELPSGKYG CCPMPNATCC SDHLHCCPQD TVCDLIQSKC LSKENATTDL LTKLPAHTVG DVKCDMEVSC PDGYTCCRLQ SGAWGCCPFT


    Database link: P28799

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HL60, PC12, MCF7, THP1, HepG2 and 293T cell line lysates; Mouse kidney, liver and spleen tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab191211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 64 kDa.

Target

  • Function

    Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
    Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.

  • Tissue specificity

    In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

  • Involvement in disease

    Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.

  • Sequence similarities

    Belongs to the granulin family.

  • Post-translational
    modifications

    Granulins are disulfide bridged.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Acrogranin antibody
    • CLN11 antibody
    • GEP antibody
    • GP88 antibody
    • Granulin A antibody
    • Granulin B antibody
    • Granulin C antibody
    • Granulin D antibody
    • Granulin E antibody
    • Granulin epithelin antibody
    • Granulin F antibody
    • Granulin G antibody
    • Granulin-7 antibody
    • Granulins antibody
    • GRN antibody
    • GRN_HUMAN antibody
    • PC cell derived growth factor antibody
    • PCDGF antibody
    • PEPI antibody
    • PGRN antibody
    • Proepithelin antibody
    • Progranulin antibody
    see all

Images

  • Western blot - Anti-Granulin antibody (ab191211)
    Western blot - Anti-Granulin antibody (ab191211)
    All lanes : Anti-Granulin antibody (ab191211) at 1/1000 dilution

    Lane 1 : HL60 cell line lysate
    Lane 2 : THP1 cell line lysate
    Lane 3 : PC12 cell line lysate
    Lane 4 : 293T cell line lysate
    Lane 5 : HepG2 cell line lysate
    Lane 6 : MCF7 cell line lysate
    Lane 7 : Mouse liver tissue lysate
    Lane 8 : Mouse spleen tissue lysate
    Lane 9 : Mouse kidney tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG (H+L)

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 5 seconds


    Blocking buffer: 3% nonfat dry milk in TBST.

References

This product has been referenced in:

  • Zhao XA  et al. Emodin Alleviates Liver Fibrosis of Mice by Reducing Infiltration of Gr1hi Monocytes. Evid Based Complement Alternat Med 2018:5738101 (2018). Read more (PubMed: 29743924) »
  • Xie W  et al. miR-34b-5p inhibition attenuates lung inflammation and apoptosis in an LPS-induced acute lung injury mouse model by targeting progranulin. J Cell Physiol 233:6615-6631 (2018). Read more (PubMed: 29150939) »
See all 3 Publications for this product

Publishing research using ab191211? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab191211.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com