Key features and details
- Rabbit polyclonal to Granulin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Granulin antibody
See all Granulin primary antibodies
DescriptionRabbit polyclonal to Granulin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide derived from the C-terminal region of human Granulin.
- Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects bands varying from approximately 64-88 kDa depending on degree of protein glycosylation.(predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGranulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
Tissue specificityIn myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
Involvement in diseaseDefects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
Sequence similaritiesBelongs to the granulin family.
modificationsGranulins are disulfide bridged.
- Information by UniProt
- Acrogranin antibody
- CLN11 antibody
- GEP antibody
All lanes : Anti-Granulin antibody (ab53880) at 1 µg/ml
Lane 1 : MDA-MB-231 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HT1080 cell lysate
Predicted band size: 64 kDa
Observed band size: 64 kDa
Detects bands varying from approximately 64-88 kDa depending on degree of protein glycosylation.
ab53880 has not yet been referenced specifically in any publications.