Recombinant Anti-Granulin antibody [EPR18539-59] (ab187070)
![Western blot - Anti-Granulin antibody [EPR18539-59] (ab187070)](https://www.abcam.com/ps/products/187/ab187070/Images/ab187070-290983-anti-granulin-antibody-epr18539-59-western-blot.jpg "Western blot - Anti-Granulin antibody [EPR18539-59] (ab187070)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18539-59] to Granulin
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Granulin antibody [EPR18539-59]
See all Granulin primary antibodies -
Description
Rabbit monoclonal [EPR18539-59] to Granulin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen tissue lysate; J774A.1 whole cell lysate. IHC-P: Mouse lung tissue. ICC/IF: J774A.1 cells. Flow Cyt (intra): J774A.1 cells. IP: J774A.1 whole cell lysate.
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General notes
ab187070 is also recommended for human and rat species for IHC.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18539-59 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
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| WB |
1/1000. Predicted molecular weight: 63 kDa.
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| IP |
1/30.
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| Notes |
|---|
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Flow Cyt (Intra)
1/1000. |
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WB
1/1000. Predicted molecular weight: 63 kDa. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
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IP
1/30. |
Target
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Function
Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. -
Tissue specificity
In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. -
Involvement in disease
Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. -
Sequence similarities
Belongs to the granulin family. -
Post-translational
modificationsGranulins are disulfide bridged. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 14824 Mouse
- SwissProt: P28798 Mouse
- Unigene: 1568 Mouse
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Alternative names
- Acrogranin antibody
- CLN11 antibody
- GEP antibody
see all
Images
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Western blot - Anti-Granulin antibody [EPR18539-59] (ab187070)Lane 1 : Anti-Granulin antibody [EPR18539-59] (ab187070) at 1/1000 dilution
Lane 2 : Anti-Granulin antibody [EPR18539-59] (ab187070) at 1/5000 dilution
Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage cell line) whole cell lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 3 seconds.
Lane 2: 10 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 23972823). Granulin (Progranulin, PGRN) is a 593-amino acid, 68.5-kDa cysteine-rich protein that is typically secreted in a highly glycosylated 88-kDa form.
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![Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (ab187070)](https://www.abcam.com/ps/products/187/ab187070/Images/ab187070-290987-anti-granulin-antibody-epr18539-59-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (ab187070)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) cells labeling Granulin with ab187070 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on J774A.1 cell line.
The nuclear counter statin is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Negative control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Flow Cytometry (Intracellular) - Anti-Granulin antibody [EPR18539-59] (ab187070)](https://www.abcam.com/ps/products/187/ab187070/Images/ab187070-290988-anti-granulin-antibody-epr18539-59-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Granulin antibody [EPR18539-59] (ab187070)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) cell line labeling Granulin with ab187070 at 1/1000 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Granulin antibody [EPR18539-59] (ab187070)](https://www.abcam.com/ps/products/187/ab187070/Images/ab187070-290989-anti-granulin-antibody-epr18539-59-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Granulin antibody [EPR18539-59] (ab187070)Granulin was immunoprecipitated from 0.35 mg of J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate with ab187070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187070 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: J774A.1 lysate 10 μg (Input).
Lane 2: ab187070 IP in J774A.1 lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187070 in J744A.1 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granulin antibody [EPR18539-59] (ab187070)](https://www.abcam.com/ps/products/187/ab187070/Images/ab187070-290994-anti-granulin-antibody-epr18539-59-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granulin antibody [EPR18539-59] (ab187070)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Granulin with ab187070 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Sporadic cytoplasmic staining in mouse lung (PMID: 23959579) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-Granulin antibody [EPR18539-59] (ab187070)](https://www.abcam.com/ps/products/187/ab187070/Images/ab187070-9-benefits-of-recombinant-antibodies.png)
Anti-Granulin antibody [EPR18539-59] (ab187070)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab187070 has been referenced in 2 publications.
- Fang W et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8+ T cell exclusion. J Exp Clin Cancer Res 40:4 (2021). WB ; Mouse . PubMed: 33390170
- Xu B et al. Abnormal angiogenesis of placenta in progranulin-deficient mice. Mol Med Rep 22:3482-3492 (2020). WB, IHC-P ; Mouse . PubMed: 32945448