Product nameAnti-Granulin antibody [EPR3781]
See all Granulin primary antibodies
DescriptionRabbit monoclonal [EPR3781] to Granulin
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Granulin aa 1-100. The exact sequence is proprietary.
- WB: A431, 293T, MCF7 and HeLa cell lysates and human fetal lung and fetal kidney tissue lysates. IHC-P: Human kidney tissue. ICC/IF: Malme-3M cells. Flow Cyt: A431 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab108608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 55, 75 kDa (predicted molecular weight: 64 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionGranulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
Tissue specificityIn myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
Involvement in diseaseDefects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
Sequence similaritiesBelongs to the granulin family.
modificationsGranulins are disulfide bridged.
- Information by UniProt
- Acrogranin antibody
- CLN11 antibody
- GEP antibody
All lanes : Anti-Granulin antibody [EPR3781] (ab108608) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Human fetal lung tissue lysate
Lane 6 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
Observed band size: 55,75 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108608 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF image of ab108608 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108608, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Granulin (red) with ab108608 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
This product has been referenced in:
- Chen L et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . Read more (PubMed: 28609022) »
- Valdez C et al. Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients. Hum Mol Genet 26:4861-4872 (2017). Read more (PubMed: 29036611) »