Overview

  • Product name
    Anti-Granulocytes antibody [HIS48]
    See all Granulocytes primary antibodies
  • Description
    Mouse monoclonal [HIS48] to Granulocytes
  • Host species
    Mouse
  • Specificity
    ab3760 recognises granulocytes and erythroid cells.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    PVG rat spleen cell suspension.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 8% Fetal calf serum, 3.16% Tris HCl
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    HIS48
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20.
Flow Cyt Use 10µl for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

References

ab33760 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

The immunogen used for this antibody was not a specific peptide but rather a cell suspension from rat spleen. Unfortunately, we have not performed any detailed mapping on this clone and thus we currently do not have any empirical data to indicate which specific region or sequence is detected by this clone.

I am very sorry that on this occasion I could not be more helpful. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your inquiry. According to our records, the specificity of this clone is supported by the following papers:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783230/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793189/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435159/

I hope this information helps

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Answer

Thank you for your reply. I am sorry that I misunderstood your original email. The antibody ab33760 was generated from PVG rat spleen cell suspension and is specific to granulocytes and erythroid cells. Unfortunately I do not have any more information on the specificity of this antibody. If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam. The antibody ab33760, anti-Granulocytes antibody [HIS48] was generated from PVG rat spleen cell suspensions. The exact epitope sequence is proprietary and I do not have access to that information and therefore I cannot tell you the exact sequence. I am sorry that I am unable to assist you further in this matter but if there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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