Anti-Granulocytes antibody [SPM250] (ab75701)
Key features and details
- Mouse monoclonal [SPM250] to Granulocytes
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Granulocytes antibody [SPM250]
See all Granulocytes primary antibodies -
Description
Mouse monoclonal [SPM250] to Granulocytes -
Host species
Mouse -
Specificity
ab75701 reacts with early precursor and mature forms of human myeloid cells. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Granulocytes. Nuclei from pokeweed mitogen stimulated peripheral blood lymphocytes.
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Positive control
- Human tonsil or lymph node tissue
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General notes
ab75701 can be useful for the detection of myeloid leukemias and granulocytic sarcomas and also can be used as a marker of granulocytes in normal tissues or inflammatory processes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: BSA, 0.0268% PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab75701 can be useful for the detection of myeloid leukemias and granulocytic sarcomas and also can be used as a marker of granulocytes in normal tissues or inflammatory processes. -
Clonality
Monoclonal -
Clone number
SPM250 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50.
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Notes |
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IHC-P
1/50. |
Target
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Relevance
Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
Images
Datasheets and documents
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Datasheet download
References (1)
ab75701 has been referenced in 1 publication.
- Kawamoto T et al. Overexpression and gene amplification of EGFR, HER2, and HER3 in biliary tract carcinomas, and the possibility for therapy with the HER2-targeting antibody pertuzumab. J Gastroenterol 50:467-79 (2015). PubMed: 25112701