Overview

  • Product name

  • Description

    Rabbit polyclonal to Granuphilin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Granuphilin (internal sequence). The exact sequence is proprietary.
    Database link: Q96C24

  • Positive control

    • WB: HEK-293T, A431 and HeLa whole cell extracts. IHC-P: Mouse pancreas and ovary tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner.
  • Sequence similarities

    Contains 2 C2 domains.
    Contains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • Cellular localization

    Membrane. Cell membrane. Cytoplasmic vesicle > secretory vesicle membrane. Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp451P0116 antibody
    • Exophilin-2 antibody
    • FLJ40960 antibody
    • Granuphilin antibody
    • SLP4 antibody
    • Synaptotagmin like 4 antibody
    • Synaptotagmin-like protein 4 antibody
    • Sytl4 antibody
    • SYTL4_HUMAN antibody
    see all

Images

  • Paraffin-embedded mouse ovary tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Granuphilin antibody (ab228892) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 76 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse pancreas tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.

References

ab228892 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228892.
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