Product nameAnti-Granuphilin antibody
See all Granuphilin primary antibodies
DescriptionRabbit polyclonal to Granuphilin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human Granuphilin (internal sequence). The exact sequence is proprietary.
Database link: Q96C24
- WB: HEK-293T, A431 and HeLa whole cell extracts. IHC-P: Mouse pancreas and ovary tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 76 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionModulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner.
Sequence similaritiesContains 2 C2 domains.
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain.
Cellular localizationMembrane. Cell membrane. Cytoplasmic vesicle > secretory vesicle membrane. Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles.
- Information by UniProt
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Paraffin-embedded mouse ovary tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-Granuphilin antibody (ab228892) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 76 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse pancreas tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.
ab228892 has not yet been referenced specifically in any publications.