Recombinant Anti-Granzyme A antibody [EPR20161] (ab209205)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)](https://www.abcam.com/ps/products/209/ab209205/Images/ab209205-270024-anti-granzyme-a-antibody-epr20161-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20161] to Granzyme A
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Granzyme A antibody [EPR20161]
See all Granzyme A primary antibodies -
Description
Rabbit monoclonal [EPR20161] to Granzyme A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human spleen, tonsil, lymph node and lung lysates. IHC-P: Human NK/T lymphoma, spleen and endometrium tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20161 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
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Database links
- Entrez Gene: 3001 Human
- Omim: 140050 Human
- SwissProt: P12544 Human
- Unigene: 90708 Human
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Alternative names
- CTL tryptase antibody
- CTLA3 antibody
- Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)
Immunohistochemical analysis of paraffin-embedded human NK/T lymphoma tissue labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human NK/T lymphoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Granzyme A antibody [EPR20161] (ab209205)](https://www.abcam.com/ps/products/209/ab209205/Images/ab209205-270026-anti-granzyme-a-antibody-epr20161-western-blot.jpg)
Western blot - Anti-Granzyme A antibody [EPR20161] (ab209205)All lanes : Anti-Granzyme A antibody [EPR20161] (ab209205) at 1/1000 dilution
Lane 1 : Human spleen lysate at 10 µg
Lane 2 : Human tonsil lysate at 10 µg
Lane 3 : Human lymph node lysate at 10 µg
Lane 4 : Human lung lysate at 20 µg
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds, Lane 2: 3 minutes, Lane 3: 10 seconds, Lane 4: 5 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)](https://www.abcam.com/ps/products/209/ab209205/Images/ab209205-270023-anti-granzyme-a-antibody-epr20161-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human spleen is observed [PMID: 9637500].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)](https://www.abcam.com/ps/products/209/ab209205/Images/ab209205-270022-anti-granzyme-a-antibody-epr20161-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (ab209205)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on some stromal cells of human endometrium is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Granzyme A antibody [EPR20161] (ab209205)](https://www.abcam.com/ps/products/209/ab209205/Images/ab209205-8-benefits-of-recombinant-antibodies.png)
Anti-Granzyme A antibody [EPR20161] (ab209205)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab209205 has been referenced in 2 publications.
- Kolt S et al. Detection of Active Granzyme A in NK92 Cells with Fluorescent Activity-Based Probe. J Med Chem 63:3359-3369 (2020). PubMed: 32142286
- Wakiyama H et al. Cytolytic Activity (CYT) Score Is a Prognostic Biomarker Reflecting Host Immune Status in Hepatocellular Carcinoma (HCC). Anticancer Res 38:6631-6638 (2018). PubMed: 30504371