Anti-Granzyme A antibody [GA6] (ab10870)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Granzyme A antibody [GA6]
See all Granzyme A primary antibodies -
Description
Mouse monoclonal [GA6] to Granzyme A -
Host species
Mouse -
Tested applications
Suitable for: IHC-FoFr, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant Granzyme A (Human).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified IgG prepared by affinity chromatography from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
GA6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab10870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19827750 | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 31.4 kDa. Recognises a 60kD disulphide-linked homodimeric protein. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
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Database links
- Entrez Gene: 3001 Human
- Omim: 140050 Human
- SwissProt: P12544 Human
- Unigene: 90708 Human
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Alternative names
- CTL tryptase antibody
- CTLA3 antibody
- Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [GA6] (ab10870)](https://www.abcam.com/ps/products/10/ab10870/Images/ab10870-235542-anti-granzyme-a-antibody-ga6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [GA6] (ab10870)This image is courtesy of an anonymous Abreviewab10870 staining Granzyme A in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-mouse IgG polyclonal (1/375) was used as the secondary antibody.
Datasheets and documents
References
This product has been referenced in:
- Li H et al. Glucocorticoid Receptor and Sequential P53 Activation by Dexamethasone Mediates Apoptosis and Cell Cycle Arrest of Osteoblastic MC3T3-E1 Cells. PLoS One 7:e37030 (2012). WB ; Mouse . Read more (PubMed: 22719835) »
- Hirono S et al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009). IHC-FoFr ; Human . Read more (PubMed: 19817750) »
