The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19827750
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration. Predicted molecular weight: 31.4 kDa.
Recognises a 60kD disulphide-linked homodimeric protein.
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis.
Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody
Cytotoxic T lymphocyte associated serine esterase 3 antibody
Cytotoxic T lymphocyte proteinase 1 antibody
Cytotoxic T-lymphocyte proteinase 1 antibody
Fragmentin 1 antibody
Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 antibody
Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) antibody
Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) antibody
Granzyme A antibody
Granzyme A precursor antibody
H factor antibody
Hanukah factor serine protease antibody
Hanukkah factor antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [GA6] (ab10870)This image is courtesy of an anonymous Abreview
ab10870 staining Granzyme A in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-mouse IgG polyclonal (1/375) was used as the secondary antibody.
Li H et al. Glucocorticoid Receptor and Sequential P53 Activation by Dexamethasone Mediates Apoptosis and Cell Cycle Arrest of Osteoblastic MC3T3-E1 Cells. PLoS One7:e37030 (2012).
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Hirono S et al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009).
Read more (PubMed: 19817750) »