Overview

  • Product name
    Granzyme B Activity Assay Kit (Fluorometric)
    See all Granzyme B kits
  • Sample type
    Cell Lysate
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    In Abcam's Granzyme B Activity Assay Kit (Fluorometric), active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

  • Notes

    Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

     

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    AFC Standard Yellow 1 x 0.1ml
    Granzyme B Assay Buffer WM 1 x 25ml
    Granzyme B Positive Control Green 1 vial
    Granzyme B Substrate Red 1 x 0.5ml
  • Research areas
  • Function
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • Sequence similarities
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt
  • Alternative names
    • C11
    • Cathepsin G-like 1
    • CTLA-1
    • CTSGL1
    • Cytotoxic T-lymphocyte proteinase 2
    • Fragmentin-2
    • GRAB_HUMAN
    • Granzyme B
    • Granzyme-2
    • Gzmb
    • Hlp
    • Human lymphocyte protein
    • Lymphocyte protease
    • SECT
    • T-cell serine protease 1-3E
    see all

Images

Protocols

References

ab157403 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Due to proprietary restrictions I cannot disclose what exactly the substrate is in this kit, but I can assure you that it is granzyme B specific.


Here is the protocol for processing cells for this assay:

For cell samples: Start with ˜2X106 cells, suspend the cell pellet 500 μl (or ˜4 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed by viewing the cells under the microscope. Spin down the sample and collect the supernatant. Use the supernatant for your subsequent assays. Appropriate dilutions of the sample must be tested in order to ensure the readings will fall within the linear range of the standard curve.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up