Granzyme B Activity Assay Kit (Fluorometric) (ab157403)

Overview

  • Product name
    Granzyme B Activity Assay Kit (Fluorometric)
    See all Granzyme B kits
  • Sample type
    Cell Lysate
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    In Abcam's Granzyme B Activity Assay Kit (Fluorometric), active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

  • Notes

    Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

     

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    AFC Standard Yellow 1 x 0.1ml
    Granzyme B Assay Buffer WM 1 x 25ml
    Granzyme B Positive Control Green 1 vial
    Granzyme B Substrate Red 1 x 0.5ml
  • Research areas
  • Function
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • Sequence similarities
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt
  • Alternative names
    • C11
    • Cathepsin G-like 1
    • CTLA-1
    • CTSGL1
    • Cytotoxic T-lymphocyte proteinase 2
    • Fragmentin-2
    • GRAB_HUMAN
    • Granzyme B
    • Granzyme-2
    • Gzmb
    • Hlp
    • Human lymphocyte protein
    • Lymphocyte protease
    • SECT
    • T-cell serine protease 1-3E
    see all

Images

Protocols

References

ab157403 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Due to proprietary restrictions I cannot disclose what exactly the substrate is in this kit, but I can assure you that it is granzyme B specific.


Here is the protocol for processing cells for this assay:

For cell samples: Sta...

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