Product nameAnti-Granzyme B antibody
See all Granzyme B primary antibodies
DescriptionRabbit polyclonal to Granzyme B
The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig, Common marmoset
Synthetic peptide within Human Granzyme B aa 1-100. The exact sequence is proprietary.
Database link: P10144
- IHC-P: Rat spinal cord tissue. Human tonsil tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19124737|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22373353
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration.|
FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
Cytotoxic effector cells and regulatory T cells in GBM xenografts growing in immunocompetent rats.
Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.
Immunohistochemical analysis of lung tissue from patients with idiopathic pulmonary fibrosis, staining Granzyme B with ab4059.
Tissue was fixed using paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% donkey serum in PBS containing 3% BSA for 45 minutes. Slides were incubated overnight at 4°C with primary antibody (1/100 in blocking solution). An AlexaFlour®568-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ab4059 at 1/20 dilution staining rat spinal cord tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 2 hours. A biotinylated goat anti-rabbit IgG was used as the secondary.
Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).
This product has been referenced in:
- Chen D et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun 9:873 (2018). Read more (PubMed: 29491374) »
- Stover DG et al. Phase II study of ruxolitinib, a selective JAK1/2 inhibitor, in patients with metastatic triple-negative breast cancer. NPJ Breast Cancer 4:10 (2018). Read more (PubMed: 29761158) »