Product nameAnti-Granzyme B antibody
See all Granzyme B primary antibodies
DescriptionRabbit polyclonal to Granzyme B
SpecificityDetects endogenous levels of total Granzyme B protein. Due to sequence homology this antibody is predicted to cross-react with granzyme H.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Human)
- NIH/3T3 cell extract or Human breast carcinoma tissue IF/ICC - methanol fixed MEF1 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use a concentration of 50 - 100 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.
All lanes : Anti-Granzyme B antibody (ab53097) at 1/300 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunizing peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
ICC/IF image of ab53097 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Sun Y et al. Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice. Exp Ther Med 12:3773-3777 (2016). Read more (PubMed: 28105109) »
- Mohamedali KA et al. The functionalized human serine protease granzyme B/VEGF121 targets tumor vasculature and ablates tumor growth. Mol Cancer Ther 12:2055-66 (2013). ICC/IF . Read more (PubMed: 23858102) »