Recombinant Anti-Granzyme B antibody [EPR20129-217] (ab208586)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266747-anti-granzyme-b-antibody-epr20129-217-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20129-217] to Granzyme B
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Granzyme B antibody [EPR20129-217]
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [EPR20129-217] to Granzyme B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Granzyme B aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P10144 -
Positive control
- WB: Human Granzyme B recombinant protein; NK-92 whole cell lysate. IHC-P: Human colon and cervix cancer tissues. ICC/IF: NK-92 cells. Flow Cyt: NK-92 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20129-217 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab208586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). | |
| ICC/IF | 1/100. | |
| Flow Cyt | 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Database links
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling Granzyme B with ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on neutrophils and stroma cells of human cervix cancer is observed [PMID: 14512315]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266746-anti-granzyme-b-antibody-epr20129-217-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (Human peripheral blood malignant Non-Hodgkin's lymphoma cell line) cells labeling Granzyme B with ab208586 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NK-92 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266750-anti-granzyme-b-antibody-epr20129-217-western-blot.jpg)
Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)All lanes : Anti-Granzyme B antibody [EPR20129-217] (ab208586) at 1/10000 dilution
Lane 1 : Human Granzyme B recombinant protein
Lane 2 : Human Granzyme H recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human Granzyme B and human Granzyme H recombinant protein contain aa21-247 and aa21-246 with a His-tag. These two recombinant proteins were made in house.
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![Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266749-anti-granzyme-b-antibody-epr20129-217-western-blot.jpg)
Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Anti-Granzyme B antibody [EPR20129-217] (ab208586) at 1/10000 dilution + NK-92 (Human peripheral blood malignant Non-Hodgkin's lymphoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 second -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266748-anti-granzyme-b-antibody-epr20129-217-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Granzyme B with ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on some stromal cells of human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266745-anti-granzyme-b-antibody-epr20129-217-flow-cytometry.jpg)
Flow Cytometry - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Flow cytometric analysis of 4% paraformaldehyde-fixed NK-92 (Human peripheral blood malignant Non-Hodgkin's lymphoma cell line) cells labeling Granzyme B with ab208586 at 1/500 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control(ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-7-benefits-of-recombinant-antibodies.png)
Anti-Granzyme B antibody [EPR20129-217] (ab208586)
Protocols
References (0)
ab208586 has not yet been referenced specifically in any publications.
