Recombinant Anti-Granzyme B antibody [EPR20129-217] (ab208586)
Rabbit recombinant monoclonal Granzyme B antibody [EPR20129-217]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Human. Immunogen corresponding to recombinant full length protein.
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Granzyme B antibody [EPR20129-217]
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [EPR20129-217] to Granzyme B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Granzyme B aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P10144 -
Positive control
- WB: Human Granzyme B recombinant protein; NK-92 whole cell lysate. IHC-P: Human colon and cervix cancer tissues. ICC/IF: NK-92 cells. Flow Cyt: NK-92 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20129-217 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab208586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). | |
| ICC/IF | 1/100. | |
| Flow Cyt | 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Database links
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266747-anti-granzyme-b-antibody-epr20129-217-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling Granzyme B with ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on neutrophils and stroma cells of human cervix cancer is observed [PMID: 14512315]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266746-anti-granzyme-b-antibody-epr20129-217-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (Human peripheral blood malignant Non-Hodgkin's lymphoma cell line) cells labeling Granzyme B with ab208586 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NK-92 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266750-anti-granzyme-b-antibody-epr20129-217-western-blot.jpg)
Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)All lanes : Anti-Granzyme B antibody [EPR20129-217] (ab208586) at 1/10000 dilution
Lane 1 : Human Granzyme B recombinant protein
Lane 2 : Human Granzyme H recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human Granzyme B and human Granzyme H recombinant protein contain aa21-247 and aa21-246 with a His-tag. These two recombinant proteins were made in house.
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![Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266749-anti-granzyme-b-antibody-epr20129-217-western-blot.jpg)
Western blot - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Anti-Granzyme B antibody [EPR20129-217] (ab208586) at 1/10000 dilution + NK-92 (Human peripheral blood malignant Non-Hodgkin's lymphoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 second -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266748-anti-granzyme-b-antibody-epr20129-217-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Granzyme B with ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on some stromal cells of human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-Granzyme B antibody [EPR20129-217] (ab208586)](https://www.abcam.com/ps/products/208/ab208586/Images/ab208586-266745-anti-granzyme-b-antibody-epr20129-217-flow-cytometry.jpg)
Flow Cytometry - Anti-Granzyme B antibody [EPR20129-217] (ab208586)Flow cytometric analysis of 4% paraformaldehyde-fixed NK-92 (Human peripheral blood malignant Non-Hodgkin's lymphoma cell line) cells labeling Granzyme B with ab208586 at 1/500 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control(ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Protocols
References
ab208586 has not yet been referenced specifically in any publications.
