Recombinant Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
![Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)](https://www.abcam.com/ps/products/226/ab226162/Images/ab226162-482705-AntiGranzyme-B-antibody-EPR8260-flow-cyomtery-nogfpcd16nk92.jpg "Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Granzyme B antibody [EPR8260] - BSA and Azide free
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human Hodgkins lymphoma. Human tonsil tissues; WB: KARPAS-299, SR whole cell lysates; Flow cyt: No-GFP-CD16.NK-92 cells.
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General notes
ab226162 is the carrier-free version of ab134933.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 7.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8260 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Database links
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
Flow cytometric analysis of 4% paraformaldehyde fixed No-GFP-CD16.NK-92 Human Natural Killer cells labeling Granzyme B with ab134933 at 1/100 dilution (Red) compared with an Isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG Alexa Fluor® 488 (ab150081), at 1/5000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).
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![Western blot - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)](https://www.abcam.com/ps/products/226/ab226162/Images/ab226162-469829-anti-granzyme-b-antibody-karpas-299-jurkat-sr-human.jpg)
Western blot - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)All lanes : Anti-Granzyme B antibody [EPR8260] (ab134933) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : SR (human pleural effusion lymphoblast, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 48 secondsNegative control: Jurkat (PMID:18437383, 25168906).
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)](https://www.abcam.com/ps/products/226/ab226162/Images/ab226162-386505-AntiGranzyme-B-antibody-monoclonal-antibody-EPR8260-ihcp-hodgkins-lymphoma-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)](https://www.abcam.com/ps/products/226/ab226162/Images/ab226162-386503-AntiGranzyme-B-antibody-monoclonal-antibody-EPR8260-ihcp-tonsil-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).
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![OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)](https://www.abcam.com/ps/products/226/ab226162/Images/ab226162-386713-Rabbi-monoclonal-AntiGranzyme-B-antibody-EPR8260-Kd.jpg)
OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).
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![Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)](https://www.abcam.com/ps/products/226/ab226162/Images/ab226162-5-benefits-of-recombinant-antibodies.png)
Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab226162 has not yet been referenced specifically in any publications.
