Recombinant
RabMAb

Recombinant Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Granzyme B antibody [EPR8260] - BSA and Azide free
    See all Granzyme B primary antibodies

  • Description

    Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Granzyme B aa 150-250. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human Hodgkins lymphoma. Human tonsil tissues; Flow Cyt: NK-92 cells.

  • General notes

    ab226162 is the carrier-free version of ab134933. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226162 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab226162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
WB Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • C11 antibody
      • Cathepsin G like 1 antibody
      • Cathepsin G-like 1 antibody
      • CCPI antibody
      • CGL 1 antibody
      • CGL1 antibody
      • CSP B antibody
      • CSPB antibody
      • CTLA 1 antibody
      • CTLA-1 antibody
      • CTLA1 antibody
      • CTSGL1 antibody
      • Cytotoxic serine protease B antibody
      • Cytotoxic T lymphocyte associated serine esterase 1 antibody
      • Cytotoxic T lymphocyte proteinase 2 antibody
      • Cytotoxic T-lymphocyte proteinase 2 antibody
      • Fragmentin 2 antibody
      • Fragmentin-2 antibody
      • GRAB_HUMAN antibody
      • Granzyme 2 antibody
      • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
      • Granzyme B antibody
      • Granzyme-2 antibody
      • GranzymeB antibody
      • GRB antibody
      • Gzmb antibody
      • Hlp antibody
      • Human lymphocyte protein antibody
      • Lymphocyte protease antibody
      • Protease, serine, B antibody
      • SECT antibody
      • T cell serine protease 1 3E antibody
      • T cell serine protease 1-3E antibody
      • T-cell serine protease 1-3E antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    • Flow Cytometry - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Flow Cytometry - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Flow Cytometry analysis of NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling Granzyme B with purified ab134933 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    • Other - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Other - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Equilibrium disassociation constant (KD)
      Learn more about KD 

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226162? Please let us know so that we can cite the reference in this datasheet.

    ab226162 has not yet been referenced specifically in any publications.

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