RecombinantRabMAb

Recombinant Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

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Western blot - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
  • OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
  • Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-Granzyme B antibody [EPR8260] - BSA and Azide free
    See all Granzyme B primary antibodies

  • Description

    Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human Hodgkins lymphoma. Human tonsil tissues; WB: KARPAS-299, SR whole cell lysates

  • General notes

    ab226162 is the carrier-free version of ab134933.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.

Target

  • Function

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • C11 antibody
      • Cathepsin G like 1 antibody
      • Cathepsin G-like 1 antibody
      • CCPI antibody
      • CGL 1 antibody
      • CGL1 antibody
      • CSP B antibody
      • CSPB antibody
      • CTLA 1 antibody
      • CTLA-1 antibody
      • CTLA1 antibody
      • CTSGL1 antibody
      • Cytotoxic serine protease B antibody
      • Cytotoxic T lymphocyte associated serine esterase 1 antibody
      • Cytotoxic T lymphocyte proteinase 2 antibody
      • Cytotoxic T-lymphocyte proteinase 2 antibody
      • Fragmentin 2 antibody
      • Fragmentin-2 antibody
      • GRAB_HUMAN antibody
      • Granzyme 2 antibody
      • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
      • Granzyme B antibody
      • Granzyme-2 antibody
      • GranzymeB antibody
      • GRB antibody
      • Gzmb antibody
      • Hlp antibody
      • Human lymphocyte protein antibody
      • Lymphocyte protease antibody
      • Protease, serine, B antibody
      • SECT antibody
      • T cell serine protease 1 3E antibody
      • T cell serine protease 1-3E antibody
      • T-cell serine protease 1-3E antibody
      see all

    Images

    • Western blot - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Western blot - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      All lanes : Anti-Granzyme B antibody [EPR8260] (ab134933) at 1/1000 dilution

      Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
      Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 3 : SR (human pleural effusion lymphoblast, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa


      Exposure time: 48 seconds


      This data was created using ab134933, the same clone in a different buffer formulation.

      Negative control: Jurkat (PMID:18437383, 25168906).

      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    • OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

      Equilibrium disassociation constant (KD)
      Learn more about KD 

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

    • Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
      Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226162? Please let us know so that we can cite the reference in this datasheet.

    ab226162 has not yet been referenced specifically in any publications.

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