Recombinant Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)](https://www.abcam.com/ps/products/289/ab289895/Images/ab289895-483513-IHChumantonsil.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [EPR8260] to Granzyme B - Chimeric – BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free
See all Granzyme B primary antibodies -
Description
Rat monoclonal [EPR8260] to Granzyme B - Chimeric – BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: KARPAS-299 and SR whole cell lysates. IHC-P: Human tonsil and kidney tissue. Flow cyt: No-GFP-CD16.NK-92 cells.
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General notes
ab289895 is the carrier-free version of ab289888.
This rat antibody has been engineered from a RabMAb parent antibody (ab134933). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
EPR8260 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Database links
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)
This data was developed using ab289888, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Granzyme B with ab289888 at 1/100 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human tonsil.
The section was incubated with ab289888 for 30 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
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![Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)](https://www.abcam.com/ps/products/289/ab289895/Images/ab289895-483549-8260rat-FC.jpg)
Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)This data was developed using ab289888, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized No-GFP-CD16.NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labelling Granzyme B with ab289888 at 1/50 dilution (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rat IgG Fc (DyLight 488, ab96971) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)](https://www.abcam.com/ps/products/289/ab289895/Images/ab289895-483511-8260MCArat.jpg)
Western blot - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)All lanes : Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (ab289888) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : SR (human pleural effusion lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 48 secondsThis data was developed using ab289888, the same antibody clone in a different buffer formulation.
Negative control: Jurkat (PMID:18437383, 25168906
5% NFDM/TBST was used as a blocking and diluting buffer.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)](https://www.abcam.com/ps/products/289/ab289895/Images/ab289895-483547-EPR8260RtIgG2aYR092206PS100humankidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)This data was developed using ab289888, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Granzyme B with ab289888 at 1/100 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on the glomerulus and tubule epithelia in human kidney (PMID: 28900605).
The section was incubated with ab289888 for 30 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used.
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![Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)](https://www.abcam.com/ps/products/289/ab289895/Images/ab289895-483551-Benefits-of-Recombinant-antibodies.jpg)
Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab289895 has not yet been referenced specifically in any publications.
