Key features and details
- Mouse monoclonal [GM4C1] to Granzyme B
- Suitable for: Flow Cyt, ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Granzyme B antibody [GM4C1]
See all Granzyme B primary antibodies
DescriptionMouse monoclonal [GM4C1] to Granzyme B
SpecificityAb26288 recognises granzyme B transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells. The antibody does not cross-react with the human granzymes A, K or M.
Tested applicationsSuitable for: Flow Cyt, ELISAmore details
Species reactivityReacts with: Human
Ab26288 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the granzyme B cDNA.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab26288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
ab26288 has not yet been referenced specifically in any publications.