Product nameGranzyme B Antibody Panel (EPR8260, EPR20129-217, BLR022E)
See all Granzyme B kits
Species reactivityReacts with: Human
Granzyme B Antibody Panel ab252202 contains multiple trial-sized versions of anti-human antibody clones against Granzyme B, specifically selected for high performance in IHC. This panel contains 3 recombinant rabbit monoclonal antibodies against human Granzyme B. They are provided in trial sizes to allow you to easily evaluate which is the best Granzyme B antibody for your IHC research assay needs.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
- Rabbit monoclonal [BLR022E] to Granzyme B (20 µL) ab243879
- Rabbit monoclonal [EPR20129-217] to Granzyme B (20 µL) ab208586
- Rabbit monoclonal [EPR8260] to Granzyme B (20 µL) ab134933
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab243879 - Anti-Granzyme B antibody [BLR022E] 2 x 10µl ab208586 - Anti-Granzyme B antibody [EPR20129-217] 2 x 10µl ab134933 - Anti-Granzyme B antibody [EPR8260] 2 x 10µl
FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
- Cathepsin G like 1
- Cathepsin G-like 1
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling Granzyme B with ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on neutrophils and stroma cells of human cervix cancer is observed [PMID: 14512315]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab243879 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252202 has not yet been referenced specifically in any publications.