Granzyme B Inhibitor Screening Assay Kit (ab157405)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameGranzyme B Inhibitor Screening Assay Kit
See all Granzyme B kits -
Assay typeQuantitative
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Product overview
In Abcam's Granzyme B Inhibitor Screening Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.
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Notes
Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
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Tested applicationsSuitable for: Inhibition Assaymore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests Granzyme B Assay Buffer WM 1 x 25ml Granzyme B Enzyme Green 1 vial Granzyme B Substrate Red 1 x 0.5ml Inhibitor Control (Ac-IEPD-CHO) Blue 1 x 20µl -
Research areas
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FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
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Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
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Alternative names
- C11
- Cathepsin G-like 1
- CTLA-1
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Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Inhibition Assay | Use at an assay dependent concentration. |
Images
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Inhibition of Granzyme B ActivityInhibition of Granzyme B Activity by Granzyme B Inhibitor, Ac-IEPD-CHO. Assay was performed following Kit protocol.
Protocols
References
ab157405 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"