Granzyme B Inhibitor Screening Assay Kit (ab157405)

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Inhibition of Granzyme B Activity

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader

    Overview

    • Product name

      Granzyme B Inhibitor Screening Assay Kit
      See all Granzyme B kits

    • Detection method

      Fluorescent

    • Assay type

      Quantitative

    • Product overview

      In Abcam's Granzyme B Inhibitor Screening Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.

    • Notes

      Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 100 tests
      Granzyme B Assay Buffer WM 1 x 25ml
      Granzyme B Enzyme Green 1 vial
      Granzyme B Substrate Red 1 x 0.5ml
      Inhibitor Control (Ac-IEPD-CHO) Blue 1 x 20µl

    • Research areas

    • Function

      This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

    • Sequence similarities

      Belongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.

    • Cellular localization

      Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
    • Information by UniProt

    • Alternative names

      • C11
      • Cathepsin G like 1
      • Cathepsin G-like 1
      • CCPI
      • CGL 1
      • CGL1
      • CSP B
      • CSPB
      • CTLA 1
      • CTLA-1
      • CTLA1
      • CTSGL1
      • Cytotoxic serine protease B
      • Cytotoxic T lymphocyte associated serine esterase 1
      • Cytotoxic T lymphocyte proteinase 2
      • Cytotoxic T-lymphocyte proteinase 2
      • Fragmentin 2
      • Fragmentin-2
      • GRAB_HUMAN
      • Granzyme 2
      • Granzyme B
      • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
      • Granzyme-2
      • GranzymeB
      • GRB
      • Gzmb
      • Hlp
      • Human lymphocyte protein
      • Lymphocyte protease
      • Protease, serine, B
      • SECT
      • T cell serine protease 1 3E
      • T cell serine protease 1-3E
      • T-cell serine protease 1-3E
      see all

    Images

    • Inhibition of Granzyme B Activity
      Inhibition of Granzyme B Activity
      Inhibition of Granzyme B Activity by Granzyme B Inhibitor, Ac-IEPD-CHO. Assay was performed following Kit protocol.

    References (0)

    Publishing research using ab157405? Please let us know so that we can cite the reference in this datasheet.

    ab157405 has not yet been referenced specifically in any publications.

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