Product nameGranzyme B overexpression 293T lysate (whole cell)
See all Granzyme B lysates
ab94040 is a 293T cell transfected lysate in which Human Granzyme B has been transiently over-expressed using a pCMV-Granzyme B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Similarity: Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Our Abpromise guarantee covers the use of ab94040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94040 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Granzyme B antibody (ab89415) at 1/500 dilution
Lane 1 : Granzyme B 293T Transfected Lysate - Positive Control (ab94040)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94040 has not yet been referenced specifically in any publications.