Key features and details
- Rabbit polyclonal to GRAP2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-GRAP2 antibody
See all GRAP2 primary antibodies
DescriptionRabbit polyclonal to GRAP2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human GRAP2 aa 149-251.
Database link: O75791
- WB: Rat liver tissue. IHC-P: Human lymph node tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab224613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 25,38 kDa.|
FunctionInteracts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Adapter protein GRID antibody
- GADS antibody
- GADS protein antibody
Anti-GRAP2 antibody (ab224613) at 1/500 dilution + Rat liver tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25,38 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRAP2 (green) using ab224613 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human lymph node tissue stained for GRAP2 using ab224613 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224613 has not yet been referenced specifically in any publications.