RecombinantRabMAb

Recombinant Anti-GRASP1 antibody [EPR11703] (ab171940)

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Flow Cytometry - Anti-GRASP1 antibody [EPR11703] (ab171940)
  • Western blot - Anti-GRASP1 antibody [EPR11703] (ab171940)
  • Anti-GRASP1 antibody [EPR11703] (ab171940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11703] to GRASP1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GRASP1 antibody [EPR11703]
    See all GRASP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11703] to GRASP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GRASP1 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q4V328

  • Positive control

    • Recombinant Human GRASP1 protein (ab163406) can be used as a positive control in WB. Human fetal brain tissue, HepG2 cell line lysate, HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Mouse
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 96 kDa.
Flow Cyt
1/10 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 96 kDa.
Flow Cyt
1/10 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC or IP.

    • Target

    Images

    • Flow Cytometry - Anti-GRASP1 antibody [EPR11703] (ab171940)
      Flow Cytometry - Anti-GRASP1 antibody [EPR11703] (ab171940)

      Flow cytometric analysis of permeabilized Hela labeling GRASP1 with ab171940 at 1/10 (red)  or a rabbit IgG (negative) (green).

    • Western blot - Anti-GRASP1 antibody [EPR11703] (ab171940)
      Western blot - Anti-GRASP1 antibody [EPR11703] (ab171940)
      All lanes : Anti-GRASP1 antibody [EPR11703] (ab171940) at 1/10000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : HepG2 cell line lysate
      Lane 3 : HeLa cell line lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 96 kDa

    • Anti-GRASP1 antibody [EPR11703] (ab171940)
      Anti-GRASP1 antibody [EPR11703] (ab171940)

    References (1)

    Publishing research using ab171940? Please let us know so that we can cite the reference in this datasheet.

    ab171940 has been referenced in 1 publication.

    • Zhou ZW  et al. Effect of willed movement training on neurorehabilitation after focal cerebral ischemia and on the neural plasticity-associated signaling pathway. Mol Med Rep 17:1173-1181 (2018). PubMed: 29115485

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