Recombinant Anti-GRASP1 antibody [EPR11703] (ab171940)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11703] to GRASP1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-GRASP1 antibody [EPR11703]
See all GRASP1 primary antibodies -
Description
Rabbit monoclonal [EPR11703] to GRASP1 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human GRASP1 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q4V328 -
Positive control
- Recombinant Human GRASP1 protein (ab163406) can be used as a positive control in WB. Human fetal brain tissue, HepG2 cell line lysate, HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11703 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
ICC |
Human
|
IHC-P |
Mouse
|
WB |
Human
|
All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 96 kDa.
|
|
Flow Cyt |
1/10 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 96 kDa. |
Flow Cyt
1/10 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Post-translational
modificationsProteolytically cleaved by caspase-3. -
Cellular localization
Early endosome. - Information by UniProt
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Database links
- Entrez Gene: 56850 Human
- Entrez Gene: 54645 Mouse
- Entrez Gene: 116493 Rat
- Omim: 300408 Human
- SwissProt: Q4V328 Human
- SwissProt: Q8VD04 Mouse
- SwissProt: Q9JHZ4 Rat
- Unigene: 109929 Human
see all -
Alternative names
- DKFZp434P0630 antibody
- DXImx47e antibody
- GRAP1_HUMAN antibody
see all
Images
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Flow cytometric analysis of permeabilized Hela labeling GRASP1 with ab171940 at 1/10 (red) or a rabbit IgG (negative) (green).
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All lanes : Anti-GRASP1 antibody [EPR11703] (ab171940) at 1/10000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HepG2 cell line lysate
Lane 3 : HeLa cell line lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 96 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab171940 has been referenced in 1 publication.
- Zhou ZW et al. Effect of willed movement training on neurorehabilitation after focal cerebral ischemia and on the neural plasticity-associated signaling pathway. Mol Med Rep 17:1173-1181 (2018). PubMed: 29115485