Recombinant
RabMAb

Recombinant Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

Overview

  • Product name

    Anti-GRASP65 antibody [EPR12439] - C-terminal
    See all GRASP65 primary antibodies
  • Description

    Rabbit monoclonal [EPR12439] to GRASP65 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GRASP65 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BQQ3-1

  • Positive control

    • 293T, HeLa, HepG2 and MCF7 cell lysates; Human colon and uterus tissues; HepG2 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 46 kDa).

Target

  • Function

    Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
  • Sequence similarities

    Belongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications

    Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
  • Cellular localization

    Golgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ23443 antibody
    • Golgi peripheral membrane protein p65 antibody
    • Golgi phosphoprotein 5 antibody
    • Golgi reassembly and stacking protein 1 antibody
    • Golgi reassembly and stacking protein 65 kDa antibody
    • Golgi reassembly and stacking protein, 65-kD antibody
    • Golgi reassembly stacking protein 1 65kDa antibody
    • Golgi reassembly stacking protein 1 antibody
    • Golgi reassembly stacking protein of 65 kDa antibody
    • Golgi reassembly-stacking protein 1 antibody
    • Golgi reassembly-stacking protein of 65 kDa antibody
    • GOLPH 5 antibody
    • GOLPH5 antibody
    • Gorasp 1 antibody
    • GORASP1 antibody
    • GORS1_HUMAN antibody
    • GRASP 65 antibody
    • GRASP65 antibody
    • MGC118894 antibody
    • MGC118897 antibody
    • P65 antibody
    see all

Images

  • Immunofluorescent analysis of HepG2 cells, labeling GRASP65 with ab174834 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • All lanes : Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GRASP65 with ab174834 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, labeling GRASP65 with ab174834 at 1/50 dilution.

  • Flow cytometry analysis of permeabilized HeLa cells labeling GRASP65 with ab174834 at 1/10 dilution (red), or negative control rabbit IgG (green).

  • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling GRASP65, immunoprecipitated using ab174834 at 1/10 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted May 24 2018

This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 06 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (SU.86.86 (pancreatic carcinoma - liver metastasis))
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
SU.86.86 (pancreatic carcinoma - liver metastasis)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 09 2015

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