Anti-GRB 14 antibody (ab137365)
Key features and details
- Rabbit polyclonal to GRB 14
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRB 14 antibody -
Description
Rabbit polyclonal to GRB 14 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 24-391 of Human GRB 14 (Uniprot ID: Q14449).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Raji cell lines; MOLT4 and Mouse liver whole cell lysates; Human NCIN87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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| Notes |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible. |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
Target
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Relevance
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date. -
Cellular localization
Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Endosome membrane. -
Database links
- Entrez Gene: 497029 Cow
- Entrez Gene: 2888 Human
- Entrez Gene: 50915 Mouse
- Entrez Gene: 58844 Rat
- Omim: 601524 Human
- SwissProt: Q5ICW4 Cow
- SwissProt: Q14449 Human
- SwissProt: Q9JLM9 Mouse
see all -
Alternative names
- GRB14 adapter protein antibody
- Grb14 antibody
- GRB14_HUMAN antibody
- Growth factor receptor bound protein 14 antibody
Images
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Western blot - Anti-GRB 14 antibody (ab137365)Anti-GRB 14 antibody (ab137365) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Western blot - Anti-GRB 14 antibody (ab137365)Anti-GRB 14 antibody (ab137365) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB 14 antibody (ab137365)Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labelling GRB 14 with ab137365 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137365 has been referenced in 2 publications.
- Taira J & Higashimoto Y Phosphorylation of Grb14 BPS domain by GSK-3 correlates with complex forming of Grb14 and insulin receptor. J Biochem 155:353-60 (2014). PubMed: 24535599
- Kopp K et al. Grb14 Is a Negative Regulator of CEACAM3-mediated Phagocytosis of Pathogenic Bacteria. J Biol Chem 287:39158-70 (2012). WB . PubMed: 22948154