Overview

  • Product name

    Anti-GRB 14 antibody
  • Description

    Rabbit polyclonal to GRB 14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 24-391 of Human GRB 14 (Uniprot ID: Q14449).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Raji cell lines; MOLT4 and Mouse liver whole cell lysates; Human NCIN87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.

Target

  • Relevance

    The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date.
  • Cellular localization

    Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Endosome membrane.
  • Database links

  • Alternative names

    • GRB14 adapter protein antibody
    • Grb14 antibody
    • GRB14_HUMAN antibody
    • Growth factor receptor bound protein 14 antibody

Images

  • Anti-GRB 14 antibody (ab137365) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Anti-GRB 14 antibody (ab137365) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labelling GRB 14 with ab137365 at 1/500 dilution.

References

This product has been referenced in:

  • Taira J & Higashimoto Y Phosphorylation of Grb14 BPS domain by GSK-3 correlates with complex forming of Grb14 and insulin receptor. J Biochem 155:353-60 (2014). Read more (PubMed: 24535599) »
  • Kopp K  et al. Grb14 Is a Negative Regulator of CEACAM3-mediated Phagocytosis of Pathogenic Bacteria. J Biol Chem 287:39158-70 (2012). WB . Read more (PubMed: 22948154) »
See all 2 Publications for this product

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