For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    grb10-antibody-ab154029.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Adapters Cytoplasmic
Share by email

Anti-GRB10 antibody (ab154029)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-GRB10 antibody (ab154029)

    Key features and details

    • Rabbit polyclonal to GRB10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human GRB10 protein (ab152439)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-GRB10 antibody
      See all GRB10 primary antibodies
    • Description

      Rabbit polyclonal to GRB10
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 354-594 of Human GRB10 (UniProt: Q13322).

    • Positive control

      • WB: HepG2 whole cell lysate (ab7900).
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human GRB10 protein (ab152439)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab154029 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (2)
    1/500 - 1/3000. Predicted molecular weight: 67 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 67 kDa.

    Target

    • Function

      Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
    • Tissue specificity

      Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
    • Sequence similarities

      Belongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.
    • Domain

      The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
    • Post-translational
      modifications

      phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
    • Cellular localization

      Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
    • Target information above from: UniProt accession Q13322 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2887 Human
      • Omim: 601523 Human
      • SwissProt: Q13322 Human
      • Unigene: 164060 Human
      • Alternative names

        • grb 10 antibody
        • GRB IR antibody
        • grb-10 antibody
        • GRB10 adapter protein antibody
        • GRB10 adaptor protein antibody
        • GRB10 antibody
        • GRB10_HUMAN antibody
        • GRBIR antibody
        • Growth factor receptor bound protein 10 antibody
        • Growth factor receptor-bound protein 10 antibody
        • Insulin receptor binding protein antibody
        • Insulin receptor binding protein GRB IR antibody
        • Insulin receptor-binding protein Grb-IR antibody
        • IRBP antibody
        • KIAA0207 antibody
        • Maternally expressed gene 1 antibody
        • MEG1 antibody
        • RSS antibody
        see all

      Images

      • Western blot - Anti-GRB10 antibody (ab154029)
        Western blot - Anti-GRB10 antibody (ab154029)
        Anti-GRB10 antibody (ab154029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

        Predicted band size: 67 kDa



        7.5% SDS PAGE.

         

        Secondary antibody - goat anti-rabbit HRP (ab6721)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab154029? Please let us know so that we can cite the reference in this datasheet.

      ab154029 has been referenced in 2 publications.

      • Ge Q  et al. Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis. Front Physiol 9:1051 (2018). PubMed: 30131712
      • García-Palmero I  et al. The adaptors Grb10 and Grb14 are calmodulin-binding proteins. FEBS Lett 591:1176-1186 (2017). PubMed: 28295264

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-GRB10 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEPG2)
      Gel Running Conditions
      Reduced Denaturing (12.5)
      Loading amount
      20 µg
      Specification
      HEPG2
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 13 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GRB10 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
      Sample
      Mouse Cell (embryonic stem cell)
      Specification
      embryonic stem cell
      Permeabilization
      Yes - 0.1% triton x
      Fixative
      methanol/acetone
      Read More

      Abcam user community

      Verified customer

      Submitted May 19 2014

      Western blot abreview for Anti-GRB10 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      100000 cells
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Sample
      Mouse Cell lysate - whole cell (mouse embryonic stem cell)
      Specification
      mouse embryonic stem cell
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 07 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.