Overview

  • Product name
  • Description
    Rabbit polyclonal to GRB10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 354-594 of Human GRB10 (UniProt: Q13322).

  • Positive control
    • HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab154029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.

Target

  • Function
    Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • Tissue specificity
    Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
  • Sequence similarities
    Belongs to the GRB7/10/14 family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
    Contains 1 SH2 domain.
  • Domain
    The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
  • Post-translational
    modifications
    phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
  • Cellular localization
    Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • grb 10 antibody
    • GRB IR antibody
    • grb-10 antibody
    • GRB10 adapter protein antibody
    • GRB10 adaptor protein antibody
    • GRB10 antibody
    • GRB10_HUMAN antibody
    • GRBIR antibody
    • Growth factor receptor bound protein 10 antibody
    • Growth factor receptor-bound protein 10 antibody
    • Insulin receptor binding protein antibody
    • Insulin receptor binding protein GRB IR antibody
    • Insulin receptor-binding protein Grb-IR antibody
    • IRBP antibody
    • KIAA0207 antibody
    • Maternally expressed gene 1 antibody
    • MEG1 antibody
    • RSS antibody
    see all

Images

  • Anti-GRB10 antibody (ab154029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 67 kDa



    7.5% SDS PAGE.

     

    Secondary antibody - goat anti-rabbit HRP (ab6721)

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Verified customer

Submitted Nov 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol/acetone
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Submitted May 19 2014

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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