Western blot abreview for Anti-GRB10 antibody

Good
Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500

Secondary antibody

Name
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 67 kDa Non-specific: 75 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
Mouse embryonic stem cells.

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Submitted Jan 07 2014

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