Immunocytochemistry/ Immunofluorescence abreview for Anti-GRB10 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol/acetone

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: blocking buffer
Dilution
1/200

Secondary antibody

Dilution
1/500

Additional data

Additional Notes
PFA fixation also tested which gave a very similar result.

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Submitted May 19 2014

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