Overview

  • Product name

  • Description

    Rabbit polyclonal to GRB10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GRB10 aa 574-594 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    YQLNKGVLPCKLKHHCIRVAL


    Database link: Q13322

  • Positive control

    • Mouse embryo tissue, Rat lung and spleen tissues, Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab217687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • Tissue specificity

    Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
  • Sequence similarities

    Belongs to the GRB7/10/14 family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
    Contains 1 SH2 domain.
  • Domain

    The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
  • Post-translational
    modifications

    phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
  • Cellular localization

    Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
  • Information by UniProt
  • Database links

  • Alternative names

    • grb 10 antibody
    • GRB IR antibody
    • grb-10 antibody
    • GRB10 adapter protein antibody
    • GRB10 adaptor protein antibody
    • GRB10 antibody
    • GRB10_HUMAN antibody
    • GRBIR antibody
    • Growth factor receptor bound protein 10 antibody
    • Growth factor receptor-bound protein 10 antibody
    • Insulin receptor binding protein antibody
    • Insulin receptor binding protein GRB IR antibody
    • Insulin receptor-binding protein Grb-IR antibody
    • IRBP antibody
    • KIAA0207 antibody
    • Maternally expressed gene 1 antibody
    • MEG1 antibody
    • RSS antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Mouse embryo tissue, labeling GRB10 with ab217687 at 1/200 dilution, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded rat lung tissue, labeling GRB10 with ab217687 at 1/200 dilution, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded rat spleen tissue, labeling GRB10 with ab217687 at 1/300 dilution, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded rat spleen tissue, labeling GRB10 with ab217687 at 1/100 dilution, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human kidney tissue, labeling GRB10 with ab217687 at 1/100 dilution, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human kidney tissue, labeling GRB10 with ab217687 at 1/300 dilution, followed by secondary detection and DAB staining.

References

ab217687 has not yet been referenced specifically in any publications.

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