Anti-GRB2 antibody (ab111031)
Key features and details
- Rabbit polyclonal to GRB2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRB2 antibody
See all GRB2 primary antibodies -
Description
Rabbit polyclonal to GRB2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P Human -
Immunogen
Synthetic peptide, corresponding to C terminal amino acids 198-217 of Human GRB2
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Positive control
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. Human Brain, Cerebellum, Human Brain, Cortex and Human Colon, Myenteric Plexus tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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All applications |
Mouse
Rat
Cow
Xenopus laevis
Zebrafish
Catfish
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Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 535298 Cow
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Entrez Gene: 444095 Xenopus laevis
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Colon Myenteric Plexus tissue by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab111031 has been referenced in 2 publications.
- Li X et al. Hsa_circ_0002483 inhibited the progression and enhanced the Taxol sensitivity of non-small cell lung cancer by targeting miR-182-5p. Cell Death Dis 10:953 (2019). PubMed: 31844042
- Zhang Y et al. Identification of GRB2 and GAB1 coexpression as an unfavorable prognostic factor for hepatocellular carcinoma by a combination of expression profile and network analysis. PLoS One 8:e85170 (2013). IHC . PubMed: 24391994