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    grb2-antibody-ab111031.pdf

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Signal Transduction Adapters Cytoplasmic
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Anti-GRB2 antibody (ab111031)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)

Key features and details

  • Rabbit polyclonal to GRB2
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human GRB2 protein (ab116430)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GRB2 antibody
    See all GRB2 primary antibodies
  • Description

    Rabbit polyclonal to GRB2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 198-217 of Human GRB2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. Human Brain, Cerebellum, Human Brain, Cortex and Human Colon, Myenteric Plexus tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.2% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human GRB2 protein (ab85917)
  • Recombinant Protein

    • Recombinant Human GRB2 protein (ab116430)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab111031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Rat
Cow
Xenopus laevis
Zebrafish
Catfish
Application Abreviews Notes
IHC-P
Use a concentration of 10 µg/ml.
Notes
IHC-P
Use a concentration of 10 µg/ml.

Target

  • Function

    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities

    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain

    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization

    Golgi apparatus.
  • Target information above from: UniProt accession P62993 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 535298 Cow
    • Entrez Gene: 2885 Human
    • Entrez Gene: 14784 Mouse
    • Entrez Gene: 81504 Rat
    • Entrez Gene: 444095 Xenopus laevis
    • Omim: 108355 Human
    • SwissProt: P62993 Human
    • SwissProt: Q60631 Mouse
    • SwissProt: P62994 Rat
    • SwissProt: Q6GPJ9 Xenopus laevis
    • Unigene: 444356 Human
    • Unigene: 708119 Human
    • Unigene: 439649 Mouse
    • Unigene: 3360 Rat
    see all
  • Alternative names

    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
    ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
    ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)
    ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Colon Myenteric Plexus tissue by Immunohistochemistry.

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab111031? Please let us know so that we can cite the reference in this datasheet.

ab111031 has been referenced in 2 publications.

  • Li X  et al. Hsa_circ_0002483 inhibited the progression and enhanced the Taxol sensitivity of non-small cell lung cancer by targeting miR-182-5p. Cell Death Dis 10:953 (2019). PubMed: 31844042
  • Zhang Y  et al. Identification of GRB2 and GAB1 coexpression as an unfavorable prognostic factor for hepatocellular carcinoma by a combination of expression profile and network analysis. PLoS One 8:e85170 (2013). IHC . PubMed: 24391994

Customer reviews and Q&As

Show All Reviews Q&A
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Question

What is the recommended antigen retrieval buffer for these three antibodies?

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Abcam community

Verified customer

Asked on Jan 23 2012

Answer

Thank you for contacting us.

The following standard heat-mediated citrate buffer antigen retrieval was used for the IHC validation of ab32111 and ab111031. Antibody ab111586 used citrate buffer in a pressure cooker at 120C for 5 minutes but a steamer or waterbath at 95-100C will be an acceptable substitute.

Place slides in a container of 0.01 M sodium citrate buffer, pH 6.0 heated to 100°C and maintain this temperature for 20 minutes by placing the container in a steamer or heated waterbath. After 20 minutes, remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 23 2012

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