- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-GRB2 antibody
See all GRB2 primary antibodies -
DescriptionRabbit polyclonal to GRB2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Catfish
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Immunogen
Synthetic peptide, corresponding to C terminal amino acids 198-217 of Human GRB2
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Positive control
- Human Brain, Cerebellum, Human Brain, Cortex and Human Colon, Myenteric Plexus tissues.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. |
Target
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FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
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Cellular localizationGolgi apparatus.
- Information by UniProt
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Database links
- Entrez Gene: 535298 Cow
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Entrez Gene: 444095 Xenopus laevis
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab111031)ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Colon Myenteric Plexus tissue by Immunohistochemistry.
References
This product has been referenced in:
- Zhang Y et al. Identification of GRB2 and GAB1 coexpression as an unfavorable prognostic factor for hepatocellular carcinoma by a combination of expression profile and network analysis. PLoS One 8:e85170 (2013). IHC . Read more (PubMed: 24391994) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"