Anti-GRB2 antibody (ab2234)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-GRB2 antibody
See all GRB2 primary antibodies -
DescriptionGoat polyclonal to GRB2
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human GRB2 aa 206-217 (C terminal).
Sequence:C-PRNYVTPVNRNV
(Peptide available asab22873) -
Positive control
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. WB: MOLT4 cell lysate, mouse, rat, human and pig spleen, mouse and rat brain, and human thymus tissue lysates. IHC-P: Human lymph node tissue.
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General notesPrincipal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab2234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. |
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| WB | Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa.Can be blocked with Human GRB2 peptide (ab22873). |
Target
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FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
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Cellular localizationGolgi apparatus.
- Information by UniProt
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Database links
- Entrez Gene: 535298 Cow
- Entrez Gene: 483312 Dog
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 100192436 Pig
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Mouse brain tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lane 3 : Mouse spleen tissue lysate in RIPA buffer
Lane 4 : Rat spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue, with no primary antibody.
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Human spleen tissue lysate in RIPA buffer
Lane 2 : Pig spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Western blot - Anti-GRB2 antibody (ab2234)Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg
Lane 1 : Human thymus tissue lysate in RIPA buffer
Lane 2 : MOLT4 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
Datasheets and documents
References
ab2234 has not yet been referenced specifically in any publications.
