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- Specific References
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Overview
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Product nameAnti-GRB2 antibody
See all GRB2 primary antibodies -
DescriptionGoat polyclonal to GRB2
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Host speciesGoat
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Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide: PRNYVTPVNRNV, corresponding to C terminal amino acids 206-217 of Human GRB2.
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General notesPrincipal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab2234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| IHC-P | ||
| WB |
Application notes
ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 0.1 - 0.3 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 0.1 - 0.3 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
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Cellular localizationGolgi apparatus.
- Information by UniProt
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Database links
- Entrez Gene: 535298 Cow
- Entrez Gene: 483312 Dog
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 100192436 Pig
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat spleen lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa -
Immunohistochemistry - Anti-GRB2 antibody (ab2234)ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue , with no primary antibody.
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Western blot - Anti-GRB2 antibody (ab2234) -
Western blot - Anti-GRB2 antibody (ab2234)Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg
Lane 1 : Human thymus lysate
Lane 2 : Molt4 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa -
Western blot - Anti-GRB2 antibody (ab2234)Anti-GRB2 antibody (ab2234) at 2 µg/ml + Jurkat lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 30 kDa
Observed band size: 30 kDa
Ab2234 staining (2µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)Ab2234 (0.3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
References
ab2234 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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