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    grb2-antibody-ab2234.pdf

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Signal Transduction Adapters Cytoplasmic
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Anti-GRB2 antibody (ab2234)

  • Datasheet
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

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Western blot - Anti-GRB2 antibody (ab2234)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)
  • Western blot - Anti-GRB2 antibody (ab2234)
  • Western blot - Anti-GRB2 antibody (ab2234)

Key features and details

  • Goat polyclonal to GRB2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GRB2 antibody [Y237] (ab32037)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GRB2 antibody
    See all GRB2 primary antibodies
  • Description

    Goat polyclonal to GRB2
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human GRB2 aa 206-217 (C terminal).
    Sequence:

    C-PRNYVTPVNRNV


    (Peptide available as ab22873)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. WB: MOLT4 cell lysate, mouse, rat, human and pig spleen, mouse and rat brain, and human thymus tissue lysates. IHC-P: Human lymph node tissue.
  • General notes

    Principal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Recombinant Human GRB2 protein (ab85917)
  • Recombinant Protein

    • Recombinant Human GRB2 protein (ab116430)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Human
All applications
Cow
Dog
Application Abreviews Notes
IHC-P
Use a concentration of 1 - 2 µg/ml.

In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.

WB (1)
Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa.

A 1 hour primary incubation is recommended for this product.

Notes
IHC-P
Use a concentration of 1 - 2 µg/ml.

In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.

WB
Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa.

A 1 hour primary incubation is recommended for this product.

Target

  • Function

    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities

    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain

    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization

    Golgi apparatus.
  • Target information above from: UniProt accession P62993 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 535298 Cow
    • Entrez Gene: 483312 Dog
    • Entrez Gene: 2885 Human
    • Entrez Gene: 14784 Mouse
    • Entrez Gene: 100192436 Pig
    • Entrez Gene: 81504 Rat
    • Omim: 108355 Human
    • SwissProt: P62993 Human
    • SwissProt: Q60631 Mouse
    • SwissProt: P62994 Rat
    • Unigene: 444356 Human
    • Unigene: 708119 Human
    • Unigene: 439649 Mouse
    • Unigene: 3360 Rat
    see all
  • Alternative names

    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • Western blot - Anti-GRB2 antibody (ab2234)
    Western blot - Anti-GRB2 antibody (ab2234)
    All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml

    Lane 1 : Mouse brain tissue lysate in RIPA buffer
    Lane 2 : Rat brain tissue lysate in RIPA buffer
    Lane 3 : Mouse spleen tissue lysate in RIPA buffer
    Lane 4 : Rat spleen tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)

    ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)

    ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue, with no primary antibody.

  • Western blot - Anti-GRB2 antibody (ab2234)
    Western blot - Anti-GRB2 antibody (ab2234)
    All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml

    Lane 1 : Human spleen tissue lysate in RIPA buffer
    Lane 2 : Pig spleen tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • Western blot - Anti-GRB2 antibody (ab2234)
    Western blot - Anti-GRB2 antibody (ab2234)
    Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
    Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg

    Lane 1 : Human thymus tissue lysate in RIPA buffer
    Lane 2 : MOLT4 cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab2234? Please let us know so that we can cite the reference in this datasheet.

    ab2234 has been referenced in 1 publication.

    • Debattisti V  et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep 29:1274-1286.e6 (2019). PubMed: 31665639

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-GRB2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Gel Running Conditions
    Reduced Denaturing (6-15%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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