Overview

  • Product name
  • Description
    Goat polyclonal to GRB2
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human GRB2 aa 206-217 (C terminal).
    Sequence:

    C-PRNYVTPVNRNV


    (Peptide available as ab22873)

  • Positive control
    • Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. WB: MOLT4 cell lysate, mouse, rat, human and pig spleen, mouse and rat brain, and human thymus tissue lysates. IHC-P: Human lymph node tissue.
  • General notes
    Principal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.

In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.

WB Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa.Can be blocked with Human GRB2 peptide (ab22873).

Target

  • Function
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities
    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain
    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml

    Lane 1 : Mouse brain tissue lysate in RIPA buffer
    Lane 2 : Rat brain tissue lysate in RIPA buffer
    Lane 3 : Mouse spleen tissue lysate in RIPA buffer
    Lane 4 : Rat spleen tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

     

     

  • ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue, with no primary antibody.

  • All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml

    Lane 1 : Human spleen tissue lysate in RIPA buffer
    Lane 2 : Pig spleen tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
    Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg

    Lane 1 : Human thymus tissue lysate in RIPA buffer
    Lane 2 : MOLT4 cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

References

ab2234 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (6-15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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