Anti-GRB2 antibody (ab2234)
Key features and details
- Goat polyclonal to GRB2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GRB2 antibody
See all GRB2 primary antibodies -
Description
Goat polyclonal to GRB2 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human GRB2 aa 206-217 (C terminal).
Sequence:C-PRNYVTPVNRNV
(Peptide available asab22873) -
Positive control
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. WB: MOLT4 cell lysate, mouse, rat, human and pig spleen, mouse and rat brain, and human thymus tissue lysates. IHC-P: Human lymph node tissue.
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General notes
Principal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Mouse
Human
|
| All applications |
Cow
Dog
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml.
In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. |
|
| WB | (1) |
Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa.
A 1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. |
|
WB
Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa. A 1 hour primary incubation is recommended for this product. |
Target
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Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 535298 Cow
- Entrez Gene: 483312 Dog
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 100192436 Pig
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Mouse brain tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lane 3 : Mouse spleen tissue lysate in RIPA buffer
Lane 4 : Rat spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue, with no primary antibody.
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Human spleen tissue lysate in RIPA buffer
Lane 2 : Pig spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Western blot - Anti-GRB2 antibody (ab2234)Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg
Lane 1 : Human thymus tissue lysate in RIPA buffer
Lane 2 : MOLT4 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
Protocols
Datasheets and documents
References (1)
ab2234 has been referenced in 1 publication.
- Debattisti V et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep 29:1274-1286.e6 (2019). PubMed: 31665639
Customer reviews and Q&As
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