Overview

  • Product name

    Anti-GRB2 antibody - C-terminal
    See all GRB2 primary antibodies
  • Description

    Rabbit polyclonal to GRB2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide within Human GRB2 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: P62993

  • Positive control

    • IHC-P: Human breast carcinoma, colon carcinoma and ovarian carcinoma tissues; Mouse liver and lung tissues; Rat fore brain tissue. WB: HEK-293T, A431, HeLa, and HepG2 whole cell extracts; NIH/3T3 and BCL-1 whole cell lysates. ICC/IF: SK-N-SH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities

    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain

    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization

    Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • All lanes : Anti-GRB2 antibody - C-terminal (ab226951) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded human breast carcinoma tissue stained for GRB2 using ab226951 at 1/500 dilution in immunohistochemical analysis.

  • SK-N-SH (human neuroblastoma cell line) cells stained for GRB2 (green) using ab226951 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded human colon carcinoma tissue stained for GRB2 using ab226951 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human ovarian carcinoma tissue stained for GRB2 using ab226951 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse liver tissue stained for GRB2 using ab226951 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse lung tissue stained for GRB2 using ab226951 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat fore brain tissue stained for GRB2 using ab226951 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-GRB2 antibody - C-terminal (ab226951) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 25 kDa

References

ab226951 has not yet been referenced specifically in any publications.

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